000135374 001__ 135374
000135374 005__ 20190316234524.0
000135374 02470 $$2PMID$$a18505770
000135374 0247_ $$2doi$$a10.1152/physiolgenomics.90207.2008
000135374 037__ $$aARTICLE
000135374 245__ $$aReliability, robustness, and reproducibility in mouse behavioral phenotyping: a cross-laboratory study
000135374 269__ $$a2008
000135374 260__ $$c2008
000135374 336__ $$aJournal Articles
000135374 520__ $$aEstablishing standard operating procedures (SOPs) as tools for the analysis of behavioral phenotypes is fundamental to mouse functional genomics. It is essential that the tests designed provide reliable measures of the process under investigation but most importantly that these are reproducible across both time and laboratories. For this reason, we devised and tested a set of SOPs to investigate mouse behavior. Five research centers were involved across France, Germany, Italy, and the UK in this study, as part of the EUMORPHIA program. All the procedures underwent a cross-validation experimental study to investigate the robustness of the designed protocols. Four inbred reference strains (C57BL/6J, C3HeB/FeJ, BALB/cByJ, 129S2/SvPas), reflecting their use as common background strains in mutagenesis programs, were analyzed to validate these tests. We demonstrate that the operating procedures employed, which includes open field, SHIRPA, grip-strength, rotarod, Y-maze, prepulse inhibition of acoustic startle response, and tail flick tests, generated reproducible results between laboratories for a number of the test output parameters. However, we also identified several uncontrolled variables that constitute confounding factors in behavioral phenotyping. The EUMORPHIA SOPs described here are an important start-point for the ongoing development of increasingly robust phenotyping platforms and their application in large-scale, multicentre mouse phenotyping programs.
000135374 6531_ $$aInternational Cooperation
000135374 6531_ $$aLaboratory Techniques and Procedures
000135374 700__ $$aMandillo, Silvia
000135374 700__ $$aTucci, Valter
000135374 700__ $$aHölter, Sabine M
000135374 700__ $$aMeziane, Hamid
000135374 700__ $$aBanchaabouchi, Mumna Al
000135374 700__ $$aKallnik, Magdalena
000135374 700__ $$aLad, Heena V
000135374 700__ $$aNolan, Patrick M
000135374 700__ $$aOuagazzal, Abdel-Mouttalib
000135374 700__ $$aCoghill, Emma L
000135374 700__ $$aGale, Karin
000135374 700__ $$aGolini, Elisabetta
000135374 700__ $$aJacquot, Sylvie
000135374 700__ $$aKrezel, Wojtek
000135374 700__ $$aParker, Andy
000135374 700__ $$aRiet, Fabrice
000135374 700__ $$aSchneider, Ilka
000135374 700__ $$aMarazziti, Daniela
000135374 700__ $$0240040$$g185233$$aAuwerx, Johan
000135374 700__ $$aBrown, Steve D M
000135374 700__ $$aChambon, Pierre
000135374 700__ $$aRosenthal, Nadia
000135374 700__ $$aTocchini-Valentini, Glauco
000135374 700__ $$aWurst, Wolfgang
000135374 773__ $$j34$$tPhysiological genomics$$k3$$q243-55
000135374 8564_ $$zURL
000135374 8564_ $$uhttps://infoscience.epfl.ch/record/135374/files/135374.pdf$$zn/a$$s1090826
000135374 909C0 $$xU11905$$0252023$$pNCEM
000135374 909CO $$ooai:infoscience.tind.io:135374$$qGLOBAL_SET$$pSV$$particle
000135374 937__ $$aNCEM-ARTICLE-2008-004
000135374 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000135374 980__ $$aARTICLE