High volume confinement in two-photon fluorescence correlation spectroscopy with radially polarized light

We present the results on two-photon total-internal-reflection fluorescence correlation spectroscopy. The combination of liquid crystal spatial light modulator, providing radial polarization, with ultrafast laser (picosecond Nd:GdVO4 laser) allowed us to take the advantage of nonlinear optical contrast mechanisms to suppress the side-lobe energy specific for radial polarization and reduce the effective excited volume twice compared to one-photon evanescent wave excitation in fluorescence correlation spectroscopy.


Published in:
Proc. SPIE, Vol. 7185, 71850D
Presented at:
BIOS, San Jose, CA, USA, January 24, 2009
Year:
2009
Keywords:
Laboratories:




 Record created 2009-03-13, last modified 2018-07-07


Rate this document:

Rate this document:
1
2
3
 
(Not yet reviewed)