000133488 001__ 133488
000133488 005__ 20181203021432.0
000133488 0247_ $$2doi$$a10.1016/S0014-5793(99)01294-6
000133488 037__ $$aARTICLE
000133488 245__ $$aMembrane interaction of TNF is not sufficient to trigger increase in membrane conductance in mammalian cells
000133488 269__ $$a1999
000133488 260__ $$c1999
000133488 336__ $$aJournal Articles
000133488 500__ $$aDepartment of Biochemistry, Sciences II, University of Geneva, Switzerland. gisou.vandergoot@biochem.unige.ch
000133488 520__ $$aTumor necrosis factor TNF can trigger increases in membrane conductance of mammalian cells in a receptor-independent manner via its lectin-like domain. A lectin-deficient TNF mutant, lacking this activity, was able to bind to artificial liposomes in a pH-dependent manner, but not to insert into the bilayer, just like wild type TNF. A peptide mimicking the lectin-like domain, which can still trigger increases in membrane currents in cells, failed to interact with liposomes. Thus, the capacity of TNF to trigger increases in membrane conductance in mammalian cells does not correlate with its ability to interact with membranes, suggesting that the cytokine does not form channels itself, but rather interacts with endogenous ion channels or with plasma membrane proteins that are coupled to ion channels.
000133488 6531_ $$aAmino Acid Sequence
000133488 6531_ $$aAnimals
000133488 6531_ $$aCell Membrane/*metabolism
000133488 6531_ $$aChlorides/metabolism
000133488 6531_ $$aCircular Dichroism
000133488 6531_ $$aEscherichia coli
000133488 6531_ $$aHydrogen-Ion Concentration
000133488 6531_ $$aIon Channels/metabolism
000133488 6531_ $$aLiposomes/metabolism
000133488 6531_ $$aMice
000133488 6531_ $$aModels
000133488 6531_ $$aMolecular
000133488 6531_ $$aMolecular Sequence Data
000133488 6531_ $$aMutation
000133488 6531_ $$aPeptide Fragments/metabolism
000133488 6531_ $$aProtein Binding
000133488 6531_ $$aProtein Denaturation
000133488 6531_ $$aProtein Folding
000133488 6531_ $$aProtein Structure
000133488 6531_ $$aSecondary
000133488 6531_ $$aRecombinant Proteins
000133488 6531_ $$aTumor Necrosis Factor-alpha/*chemistry/genetics
000133488 700__ $$0240085$$g171549$$avan der Goot, F. G.
000133488 700__ $$aPugin, J.
000133488 700__ $$aHribar, M.
000133488 700__ $$aFransen, L.
000133488 700__ $$aDunant, Y.
000133488 700__ $$aDe Baetselier, P.
000133488 700__ $$aBloc, A.
000133488 700__ $$aLucas, R.
000133488 773__ $$j460$$tFEBS Lett$$k1$$q107-11
000133488 909C0 $$xU11271$$0252037$$pVDG
000133488 909CO $$pSV$$particle$$ooai:infoscience.tind.io:133488
000133488 937__ $$aVDG-ARTICLE-1999-003
000133488 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000133488 980__ $$aARTICLE