000131154 001__ 131154
000131154 005__ 20190601122118.0
000131154 0247_ $$2doi$$a10.1016/j.mee.2008.11.087
000131154 022__ $$a0167-9317
000131154 02470 $$2ISI$$a000267273300251
000131154 037__ $$aARTICLE
000131154 245__ $$aSandwich immunoassay on a microfluidic chip using patterns of electrostatically self-assembled streptavidin-coated beads
000131154 260__ $$bElsevier$$c2009
000131154 269__ $$a2009
000131154 336__ $$aJournal Articles
000131154 520__ $$aIn this paper, we demonstrate a bead-based microfluidic immunoassay using self-assembled streptavidin-coated bead micropatterns. The beads are assembled on the microfluidic channel surface using electrostatic forces between the negatively-charged streptavidin-coated beads and the positively-charged aminosilane micropatterns. The immunoassay is performed in the stop-flow mode and uses mouse IgG diluted in phosphate buffered saline (PBS) solution as the model target antigen. The total immunoassay takes around 30 min and is able to detect mouse IgG antigen down to a concentration of 15 ng/mL by using only 560 nl of analyte volume.
000131154 6531_ $$aLab-on-a-chip
000131154 6531_ $$aImmunoassay
000131154 6531_ $$aBeads
000131154 6531_ $$aElectrostatic self-assembly
000131154 6531_ $$aOn-Chip
000131154 6531_ $$aChannels
000131154 6531_ $$aSystems
000131154 700__ $$aSivagnanam, V.
000131154 700__ $$aBouhmad, A.
000131154 700__ $$0241033$$g156714$$aLacharme, F.
000131154 700__ $$0240440$$g138412$$aVandevyver, C.
000131154 700__ $$0242801$$g113762$$aGijs, M. A. M.
000131154 773__ $$j86$$tMicroelectronic Engineering$$k4-6$$q1404-1406
000131154 909C0 $$xU10322$$0252094$$pLMIS2
000131154 909CO $$pSTI$$particle$$ooai:infoscience.tind.io:131154
000131154 917Z8 $$x104278
000131154 917Z8 $$x104278
000131154 937__ $$aLMIS2-ARTICLE-2009-005
000131154 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000131154 980__ $$aARTICLE