000130662 001__ 130662
000130662 005__ 20190316234443.0
000130662 0247_ $$2doi$$a10.1038/nmeth.1289
000130662 037__ $$aARTICLE
000130662 245__ $$aAn in vitro microfluidic approach to generating protein-interaction networks
000130662 269__ $$a2009
000130662 260__ $$c2009
000130662 336__ $$aJournal Articles
000130662 520__ $$aWe developed an in vitro protein expression and interaction analysis platform based on a highly parallel and sensitive microfluidic affinity assay, and used it for 14,792 on-chip experiments, which exhaustively measured the protein-protein interactions of 43 Streptococcus pneumoniae proteins in quadruplicate. The resulting network of 157 interactions was denser than expected based on known networks. Analysis of the network revealed previously undescribed physical interactions among members of some biochemical pathways.
000130662 700__ $$aGerber, D
000130662 700__ $$0243949$$g182445$$aMaerkl, SJ
000130662 700__ $$aQuake, SR
000130662 773__ $$tNature Methods
000130662 8564_ $$zURL
000130662 8564_ $$uhttps://infoscience.epfl.ch/record/130662/files/Gerber_2008.pdf$$zn/a$$s501452
000130662 909C0 $$xU11835$$0252240$$pLBNC
000130662 909CO $$ooai:infoscience.tind.io:130662$$qGLOBAL_SET$$pSTI$$particle
000130662 937__ $$aLBNC-ARTICLE-2008-004
000130662 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000130662 980__ $$aARTICLE