000130535 001__ 130535
000130535 005__ 20190416055730.0
000130535 02470 $$2DAR$$a12255
000130535 02470 $$2ISI$$a000252125900030
000130535 0247_ $$2doi$$a10.1093/nar/gkm912
000130535 037__ $$aARTICLE
000130535 245__ $$aSaccharomyces cerevisiae Ebs1p is a putative ortholog of human Smg7 and promotes nonsense mediated mRNA decay
000130535 269__ $$a2007
000130535 260__ $$c2007
000130535 336__ $$aJournal Articles
000130535 520__ $$aThe Smg proteins Smg5, Smg6 and Smg7 are involved in nonsense-mediated RNA decay (NMD) in metazoans, but no orthologs have been found in the budding yeast Saccharomyces cerevisiae. Sequence alignments reveal that yeast Ebs1p is similar in structure to the human Smg5-7, with highest homology to Smg7. We demonstrate here that Ebs1p is involved in NMD and behaves similarly to human Smg proteins. Indeed, both loss and overexpression of Ebs1p results in stabilization of NMD targets. However, Ebs1-loss in yeast or Smg7-depletion in human cells only partially disrupts NMD and in the latter, Smg7-depletion is partially compensated for by Smg6. Ebs1p physically interacts with the NMD helicase Upf1p and overexpressed Ebs1p leads to recruitment of Upf1p into cytoplasmic P-bodies. Furthermore, Ebs1p localizes to P-bodies upon glucose starvation along with Upf1p. Overall our findings suggest that NMD is more conserved in evolution than previously thought, and that at least one of the Smg5-7 proteins is conserved in budding yeast.
000130535 700__ $$aLuke, B.
000130535 700__ $$aAzzalin, C. M.
000130535 700__ $$aHug, N.
000130535 700__ $$aDeplazes, A.
000130535 700__ $$aPeter, M.
000130535 700__ $$0240570$$g168670$$aLingner, J.
000130535 773__ $$j35$$tNucleic Acids Res.$$k22$$q7688-7697
000130535 8564_ $$zURL
000130535 8564_ $$uhttps://infoscience.epfl.ch/record/130535/files/Luke_Nuc-Acid-Res_2007.pdf$$zn/a$$s4562307
000130535 909C0 $$xU11159$$0252147$$pUPLIN
000130535 909CO $$ooai:infoscience.tind.io:130535$$qGLOBAL_SET$$pSV$$particle
000130535 937__ $$aUPLIN-ARTICLE-2008-009
000130535 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000130535 980__ $$aARTICLE