Calmodulin-tagged phage and two-filter sandwich assays for the identification of enzymatic activities

The procedures for calmodulin-tagged phage and two-filter sandwich assays for the isolation of enzymic activities are presented. In the first method, enzymes are displayed on filamentous phage, and the reaction substrate is anchored on the calmodulin-tagged phage-enzymes using a calmodulin binding peptide. In the second method, enzyme mutants are expressed in bacterial colonies on a porous "master" filter, and the enzymes are released and diffuse to a second reaction filter that closely contacts the master filter. [on SciFinder (R)]


Published in:
Directed Enzyme Evolution, 230, 313-328
Year:
2003
Publisher:
Totowa, Humana Press
ISBN:
1-58829-286-X
Keywords:
Note:
CAN 140:212803
7-1
Enzymes
Institute of Pharmaceutical Sciences,Swiss Federal Institute of Technology,Zurich,Switz.
Journal
1064-3745
written in English.
37340-95-7 (Biotin ligase) Role: ANT (Analyte), BSU (Biological study, unclassified), ANST (Analytical study), BIOL (Biological study) (calmodulin-tagged phage and two-filter sandwich assays for identification of enzyme activities)
Laboratories:




 Record created 2008-11-17, last modified 2018-09-13


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