000128842 001__ 128842
000128842 005__ 20190316234417.0
000128842 0247_ $$2doi$$a10.1016/j.ejpb.2007.02.024
000128842 02470 $$2ISI$$a000253038300015
000128842 037__ $$aARTICLE
000128842 245__ $$aEffect of cell media on polymer coated superparamagnetic iron oxide nanoparticles (SPIONs): Colloidal stability, cytotoxicity, and cellular uptake studies
000128842 269__ $$a2008
000128842 260__ $$c2008
000128842 336__ $$aJournal Articles
000128842 520__ $$aThe influence of the composition of the polymer coated polyvinyl alcohol (PVA), vinyl alcohol/vinyl amine copolymer (A-PVA) and polyethylenimine (PEI) coated superparamagnetic iron oxide nanoparticles (SPIONs) on the colloidal stability, cytotoxicity and cellular uptake of these particles in different cell media is reported in this paper. Although all examined polymer coated SPIONs were stable in water and PBS buffer these colloidal systems had different stabilities in DMEM or RPMI media without and supplemented with fetal calf serum (FCS). We found that A-PVA coating onto the surface of the SPIONs decreased the cytotoxicity of the polymer compared to the same concentration of A-PVA alone. As well, polyplexes of PEI-SPIONs with DNA in concentration used for transfection experiments showed no cytotoxicity compared to PEI and PEI-SPIONs. Our data show that the choice of medium largely influences the uptake of these particles by HeLa cells. The optimal medium is different for the different examined polymer coated SPIONs and it should be determined in each case, individually.
000128842 6531_ $$asuperparamagnetism
000128842 6531_ $$airon oxide
000128842 6531_ $$ananoparticles
000128842 6531_ $$aPVA
000128842 6531_ $$aPEI
000128842 6531_ $$acolloidal stability
000128842 6531_ $$acytotoxicity
000128842 6531_ $$auptake
000128842 700__ $$aPetri-Fink, A.
000128842 700__ $$aSteitz, B.
000128842 700__ $$aFinka, A.
000128842 700__ $$aSalaklang, J.
000128842 700__ $$g105410$$aHofmann, H.$$0240408
000128842 773__ $$j68$$tEuropean Journal of Pharmaceutics and Biopharmaceutics$$k1$$q129-137
000128842 8564_ $$uhttps://infoscience.epfl.ch/record/128842/files/Steitz%2708-Pharmaceutics.pdf$$zn/a$$s607089$$yn/a
000128842 909C0 $$xU10324$$0252064$$pLMIS4
000128842 909CO $$ooai:infoscience.tind.io:128842$$qGLOBAL_SET$$pSTI$$particle
000128842 917Z8 $$x102085
000128842 937__ $$aLMIS4-ARTICLE-2008-026
000128842 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000128842 980__ $$aARTICLE