000128838 001__ 128838
000128838 005__ 20190416055716.0
000128838 0247_ $$2doi$$a10.1039/b811427g
000128838 02470 $$2ISI$$a000261744700010
000128838 037__ $$aARTICLE
000128838 245__ $$aTime-resolved luminescence microscopy of bimetallic lanthanide helicates in living cells
000128838 269__ $$a2008
000128838 260__ $$c2008
000128838 336__ $$aJournal Articles
000128838 520__ $$aThe cellular uptake mechanism and intracellular distribution of emissive lanthanide helicates have been elucidated by time-resolved luminescence microscopy (TRLM). The helicates are non-cytotoxic and taken up by normal (HaCat) and cancer (HeLa, MCF-7) cells by endocytosis and show a late endosomal–lysosomal cellular distribution. The lysosomes predominantly localize around the nucleus and co-localize with the endoplasmatic reticulum. The egress is slow and limited, around 30% after 24 h. The first bright luminescent images can be observed with an external concentration gradient of 5 mMof the EuIII helicate [Q = 0.21, t = 2.43 ms], compared to >10 mM when using conventional luminescence microscopy. Furthermore, multiplex labeling could be achieved with the TbIII [Q = 0.11, t = 0.65 ms], and SmIII [Q = 0.0038, t = 0.030 ms] analogues.
000128838 700__ $$0242690$$g176618$$aSong, Bo
000128838 700__ $$0240440$$g138412$$aVandevyver, Caroline D. B.
000128838 700__ $$g149479$$aChauvin, Anne-Sophie$$0240735
000128838 700__ $$aBünzli, Jean-Claude G.$$g122803$$0241990
000128838 773__ $$j6$$tOrg. Biomol. Chem.$$q4125-4133
000128838 8564_ $$uhttps://infoscience.epfl.ch/record/128838/files/OBC-08-Imaging-Song.pdf$$zn/a$$s3063869
000128838 909C0 $$xU10099$$0252081$$pLCSL
000128838 909CO $$ooai:infoscience.tind.io:128838$$qGLOBAL_SET$$pSB$$particle
000128838 937__ $$aLCSL-ARTICLE-2008-027
000128838 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000128838 980__ $$aARTICLE