000128682 001__ 128682
000128682 005__ 20180913054914.0
000128682 0247_ $$2doi$$a10.1242/jcs.021584
000128682 02470 $$2DAR$$a12634
000128682 02470 $$2ISI$$a000254660200013
000128682 037__ $$aARTICLE
000128682 245__ $$aChemical genetic analysis of the regulatory role of Cdc2p in the S. pombe septation initiation network
000128682 269__ $$a2008
000128682 260__ $$c2008
000128682 336__ $$aJournal Articles
000128682 520__ $$aThe protein kinase Cdc2p is the master regulator of cell cycle progression in the fission yeast Schizosaccharomyces pombe. It is required both for entry into mitosis and for onset of DNA replication. Cdc2p must be inactivated to permit exit from mitosis, licensing of replication origins and cytokinesis. To study the role of Cdc2p in greater detail, we generated a cdc2 allele that is sensitive to an inhibitory ATP analogue. We show that the inhibitor-induced cell cycle arrest is reversible and examine the effect of inhibiting Cdc2p on the regulation of the septation initiation network (SIN), which controls the initiation of cytokinesis in S. pombe. We found that specific inactivation of Cdc2p in a mitotically arrested cell promotes the asymmetrical recruitment of SIN proteins to the spindle poles and the recruitment of the most downstream SIN components and beta-(1,3) glucan synthase to the contractile ring. Thus, we conclude that inactivation of Cdc2p is sufficient to activate the SIN and promote cytokinesis.
000128682 6531_ $$aCDC2 Protein Kinase/antagonists &
000128682 6531_ $$ainhibitors/genetics/*physiology
000128682 6531_ $$aCell Cycle/drug effects
000128682 6531_ $$aCell Cycle Proteins/physiology
000128682 6531_ $$a*Cytokinesis
000128682 6531_ $$aMeiosis
000128682 6531_ $$aMitosis
000128682 6531_ $$aMutation
000128682 6531_ $$aProtein Kinases/metabolism
000128682 6531_ $$aProtein Tyrosine Phosphatases/physiology
000128682 6531_ $$aPyrazoles/pharmacology
000128682 6531_ $$aPyrimidines/pharmacology
000128682 6531_ $$aSchizosaccharomyces/*cytology/enzymology/genetics
000128682 6531_ $$aSchizosaccharomyces pombe Proteins/analysis/antagonists &
000128682 6531_ $$ainhibitors/genetics/*physiology
000128682 700__ $$aDischinger, S.
000128682 700__ $$0243132$$aKrapp, A.$$g181959
000128682 700__ $$aXie, L.
000128682 700__ $$aPaulson, J. R.
000128682 700__ $$0240501$$aSimanis, V.$$g174228
000128682 773__ $$j121$$kPt 6$$q843-53$$tJ Cell Sci
000128682 8564_ $$zURL
000128682 8564_ $$s1714248$$uhttps://infoscience.epfl.ch/record/128682/files/Dischinger_S.pdf$$zn/a
000128682 909C0 $$0252141$$pUPSIM$$xU11430
000128682 909CO $$ooai:infoscience.tind.io:128682$$pSV$$particle
000128682 937__ $$aUPSIM-ARTICLE-2008-003
000128682 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000128682 980__ $$aARTICLE