000125665 001__ 125665
000125665 005__ 20180317093000.0
000125665 0247_ $$2doi$$a10.1038/nprot.2008.38
000125665 037__ $$aARTICLE
000125665 245__ $$aChromatin immunoprecipitation (ChIP) coupled to detection by quantitative real-time PCR to study transcription factor binding to DNA in Caenorhabditis elegans
000125665 269__ $$a2008
000125665 260__ $$c2008
000125665 336__ $$aJournal Articles
000125665 520__ $$aIn order to determine how signaling pathways differentially regulate gene expression, it is necessary to identify the interactions between transcription factors (TFs) and their cognate cis-regulatory DNA elements. Here, we have outlined a chromatin immunoprecipitation (ChIP) protocol for use in whole Caenorhabditis elegans extracts. We discuss optimization of the procedure, including growth and harvesting of the worms, formaldehyde fixation, TF immunoprecipitation and analysis of bound sequences through real-time PCR. It takes approximately 10-12 d to obtain the worm culture for ChIP; the ChIP procedure is spaced out over a period of 2.5 d with two overnight incubations.
000125665 700__ $$aMukhopadhyay, A.
000125665 700__ $$0244717$$aDeplancke, B.$$g182376
000125665 700__ $$aWalhout, A. J.
000125665 700__ $$aTissenbaum, H. A.
000125665 773__ $$j3$$k4$$q698-709$$tNat Protoc
000125665 909CO $$ooai:infoscience.tind.io:125665$$particle$$pSV
000125665 909C0 $$0252247$$pUPDEPLA$$xU11829
000125665 937__ $$aUPDEPLA-ARTICLE-2008-001
000125665 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000125665 980__ $$aARTICLE