000125658 001__ 125658
000125658 005__ 20181203021249.0
000125658 0247_ $$2doi$$a10.1101/gr.2497604
000125658 037__ $$aARTICLE
000125658 245__ $$aA first version of the Caenorhabditis elegans Promoterome
000125658 269__ $$a2004
000125658 260__ $$c2004
000125658 336__ $$aJournal Articles
000125658 520__ $$aAn important aspect of the development of systems biology approaches in metazoans is the characterization of expression patterns of nearly all genes predicted from genome sequences. Such "localizome" maps should provide information on where (in what cells or tissues) and when (at what stage of development or under what conditions) genes are expressed. They should also indicate in what cellular compartments the corresponding proteins are localized. Caenorhabditis elegans is particularly suited for the development of a localizome map since all its 959 adult somatic cells can be visualized by microscopy, and its cell lineage has been completely described. Here we address one of the challenges of C. elegans localizome mapping projects: that of obtaining a genome-wide resource of C. elegans promoters needed to generate transgenic animals expressing localization markers such as the green fluorescent protein (GFP). To ensure high flexibility for future uses, we utilized the newly developed MultiSite Gateway system. We generated and validated "version 1.1" of the Promoterome: a resource of approximately 6000 C. elegans promoters. These promoters can be transferred easily into various Gateway Destination vectors to drive expression of markers such as GFP, alone (promoter::GFP constructs), or in fusion with protein-encoding open reading frames available in ORFeome resources (promoter::ORF::GFP).
000125658 6531_ $$aAnimals
000125658 6531_ $$aAnimals
000125658 6531_ $$aGenetically Modified
000125658 6531_ $$aCaenorhabditis elegans/genetics
000125658 6531_ $$aCloning
000125658 6531_ $$aMolecular
000125658 6531_ $$aGene Expression
000125658 6531_ $$aGenes
000125658 6531_ $$aHelminth
000125658 6531_ $$aGreen Fluorescent Proteins
000125658 6531_ $$aLuminescent Proteins/genetics
000125658 6531_ $$aOpen Reading Frames/physiology
000125658 6531_ $$aPromoter Regions (Genetics)/genetics
000125658 6531_ $$aTranscription Factors/physiology
000125658 700__ $$aDupuy, D.
000125658 700__ $$aLi, Q. R.
000125658 700__ $$0244717$$g182376$$aDeplancke, B.
000125658 700__ $$aBoxem, M.
000125658 700__ $$aHao, T.
000125658 700__ $$aLamesch, P.
000125658 700__ $$aSequerra, R.
000125658 700__ $$aBosak, S.
000125658 700__ $$aDoucette-Stamm, L.
000125658 700__ $$aHope, I. A.
000125658 700__ $$aHill, D. E.
000125658 700__ $$aWalhout, A. J.
000125658 700__ $$aVidal, M.
000125658 773__ $$j14$$tGenome Res$$k10B$$q2169-75
000125658 909C0 $$xU11829$$0252247$$pUPDEPLA
000125658 909CO $$pSV$$particle$$ooai:infoscience.tind.io:125658
000125658 937__ $$aUPDEPLA-ARTICLE-2004-001
000125658 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000125658 980__ $$aARTICLE