000125657 001__ 125657
000125657 005__ 20181203021249.0
000125657 037__ $$aARTICLE
000125657 245__ $$aMolecular ecological analysis of porcine ileal microbiota responses to antimicrobial growth promoters
000125657 269__ $$a2003
000125657 260__ $$c2003
000125657 336__ $$aJournal Articles
000125657 520__ $$aCultivation-independent microbial molecular ecology approaches were used to examine the effects of antibiotic growth promoters on the pig ileal microbiota. Five-week-old barrows were fitted with a simple T-cannula at the distal ileum. Three diets meeting or exceeding the minimum nutrient requirements were fed for 5 wk and supplemented as follows: 1) negative control (no antibiotic; n = 5), 2) continuous tylosin administration (n = 5), and 3) an antibiotic rotation sequence (wk 1, chlorotetracycline sulfathiazole penicillin; wk 2, bacitracin and roxarsone; wk 3, lincomycin; wk 4, carbadox; wk 5, virginiamycin; n = 5). Ileal luminal contents were collected for DNA isolation at the end of each of the 5 wk of the testing period. The V3 region of 16S rDNA was amplified by PCR and analyzed via denaturing gradient gel electrophoresis (DGGE) and quantitative polymerase chain reaction (qPCR). Resulting PCR-DGGE band numbers (bacterial species) were counted, and the banding patterns analyzed by calculating Sorenson's pairwise similarity coefficients (C(S)), an index measuring bacterial species in common among samples. Band numbers and total bacterial DNA concentrations decreased (P < 0.05) temporally in antibiotic-treated pigs compared with controls. Comparisons between treatments yielded low intertreatment C(S) indices, indicating treatment-dependent alterations in banding patterns, whereas intratreatment comparisons revealed increased homogeneity in antibiotic-treated vs. control pigs. Sequence analysis of treatment-specific bands identified three Lactobacillus, one Streptococcus, and one Bacillus species that were diminished with antibiotic rotation treatment, whereas tylosin selected for the presence of L. gasseri. Lactobacillus-specific qPCR was performed and analyzed as a percentage of total bacteria to further evaluate the effects of antibiotic administration on this genus. Total bacteria were decreased (P < 0.05) by tylosin and rotation treatments, whereas the percentage of lactobacilli increased (P < 0.05) by d 14 and through d 28 in tylosin-treated pigs. The decrease in total bacteria by antibiotics may reduce host-related intestinal or immune responses, which would divert energy that could otherwise be used for growth. Conversely, the ability of tylosin to improve animal growth may relate to its apparent selection for lactobacilli, commensals known to competitively exclude potentially pathogenic species from colonizing the intestine.
000125657 6531_ $$aAnimals
000125657 6531_ $$aAnti-Bacterial Agents/administration & dosage
000125657 6531_ $$aBacteria/drug effects/genetics
000125657 6531_ $$aDNA
000125657 6531_ $$aBacterial/analysis/genetics
000125657 6531_ $$aDNA
000125657 6531_ $$aRibosomal/analysis/genetics
000125657 6531_ $$aEcosystem
000125657 6531_ $$aElectrophoresis
000125657 6531_ $$aAgar Gel/methods/veterinary
000125657 6531_ $$aIleum/drug effects/microbiology
000125657 6531_ $$aMale
000125657 6531_ $$aPolymerase Chain Reaction/methods/veterinary
000125657 6531_ $$aRNA
000125657 6531_ $$aRibosomal
000125657 6531_ $$a16S/analysis
000125657 6531_ $$aRandom Allocation
000125657 6531_ $$aSpecies Specificity
000125657 6531_ $$aSwine/growth & development/microbiology
000125657 6531_ $$aTime Factors
000125657 700__ $$aCollier, C. T.
000125657 700__ $$aSmiricky-Tjardes, M. R.
000125657 700__ $$aAlbin, D. M.
000125657 700__ $$aWubben, J. E.
000125657 700__ $$aGabert, V. M.
000125657 700__ $$0244717$$g182376$$aDeplancke, B.
000125657 700__ $$aBane, D.
000125657 700__ $$aAnderson, D. B.
000125657 700__ $$aGaskins, H. R.
000125657 773__ $$j81$$tJ Anim Sci$$k12$$q3035-45
000125657 909C0 $$xU11829$$0252247$$pUPDEPLA
000125657 909CO $$pSV$$particle$$ooai:infoscience.tind.io:125657
000125657 937__ $$aUPDEPLA-ARTICLE-2003-004
000125657 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000125657 980__ $$aARTICLE