000125633 001__ 125633
000125633 005__ 20181203021249.0
000125633 02470 $$2DAR$$a13191
000125633 02470 $$2ISI$$a000257958800029
000125633 037__ $$aARTICLE
000125633 245__ $$aEpidermal Receptor Activator of NF-{kappa}B Ligand Controls Langerhans Cells Numbers and Proliferation
000125633 269__ $$a2008
000125633 260__ $$c2008
000125633 336__ $$aJournal Articles
000125633 520__ $$aLangerhans cells (LC) are the dendritic APC population of the epidermis, where they reside for long periods and are self-replicating. The molecular signals underlying these characteristics are unknown. The TNF superfamily member receptor activator of NF-kappaB ligand (RANKL, TNFSF11) has been shown to sustain viability of blood dendritic cells in addition to its role in promoting proliferation and differentiation of several cell types, notably osteoclasts. In this study, we have studied expression of the RANKL system in skin and have defined a key role for this molecule in LC homeostasis. In vitro and in vivo, human KC expressed RANKL and epidermal LC expressed cell surface RANK. In vitro, RANKL sustained CD34(+) progenitor-derived LC viability following 72-h cultures in cytokine-free medium (79.5 +/- 1% vs 55.2 +/- 5.7% live cells, respectively; n = 4; p < 0.05). In vivo, RANKL-deficient mice displayed a marked reduction in epidermal LC density (507.1 +/- 77.2 vs 873.6 +/- 41.6 LC per mm(2); n = 9; p < 0.05) and their proliferation was impaired without a detectable effect on apoptosis. These data indicate a key role for the RANKL system in the regulation of LC survival within the skin and suggest a regulatory role for KC in the maintenance of epidermal LC homeostasis.
000125633 6531_ $$areceptor activator of NF-kappaB ligand (RANKL
000125633 6531_ $$aTNFSF11)
000125633 6531_ $$aLangerhans cells
000125633 6531_ $$aepidermis
000125633 6531_ $$aTNF superfamily
000125633 6531_ $$aproliferation
000125633 700__ $$aBarbaroux, JB
000125633 700__ $$0240287$$aBeleut, M$$g174062
000125633 700__ $$0240288$$aBrisken, C$$g169115
000125633 700__ $$aMueller, CG
000125633 700__ $$aGroves, RW
000125633 773__ $$j181$$k2$$q1103-1108$$tJ Immunol.
000125633 909C0 $$0252103$$pUPBRI$$xU11257
000125633 909CO $$ooai:infoscience.tind.io:125633$$pSV$$particle
000125633 937__ $$aUPBRI-ARTICLE-2008-003
000125633 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000125633 980__ $$aARTICLE