000125305 001__ 125305
000125305 005__ 20190316234311.0
000125305 0247_ $$2doi$$a10.1117/12.728370
000125305 037__ $$aCONF
000125305 245__ $$aExtended focus Fourier domain optical coherence microscopy assists developmental biology
000125305 260__ $$bSPIE$$c2007
000125305 269__ $$a2007
000125305 336__ $$aConference Papers
000125305 520__ $$aWe present a novel detection scheme for Fourier domain optical coherence microscopy (FDOCM). A Bessel-like interference pattern with a strong central lobe was created with an axicon lens. This pattern was then imaged by a telescopic system into the sample space to obtain a laterally highly confined illumination needle, extending over a long axial range. For increased efficiency, the detection occurs decoupled from the illumination, avoiding a double pass through the axicon. Nearly constant transverse resolution of ~1.5µm along a focal range of 200µm with a maximum sensitivity of 105dB was obtained. A broad bandwidth Ti:Sapphire laser allowed for an axial resolution of 3µm in air, providing the nearly isotropic resolution necessary to access the microstructure of biological tissues. Together with the speed- and sensitivity-advantage of FDOCT, this system can perform in vivo measurements in a minimally invasive way. Tomograms of the mouse mammary gland and the mouse follicle, recorded in vitro, revealed biologically relevant structural details. Images acquired with classical microscopy techniques, involving stained and fluorescent samples, validate these structures and emphasize the high contrast of the tomograms. It is comparable to the contrast achieved with classical techniques, but employing neither staining, labeling nor slicing of the samples, stressing the high potential of FDOCM for minimally invasive in vivo small animal imaging.
000125305 700__ $$aVilliger, Martin L.
000125305 700__ $$0240287$$g174062$$aBeleut, Manfred
000125305 700__ $$0240288$$g169115$$aBrisken, Cathrin
000125305 700__ $$aLasser, Theo
000125305 700__ $$aLeitgeb, Rainer A.
000125305 7112_ $$dSunday 17 June 2007$$cMunich, Germany$$aOptical Coherence Tomography and Coherence Techniques III
000125305 773__ $$j6627$$tProceedings of SPIE: Advances in OCT System Technology II$$q66271H
000125305 8564_ $$zURL
000125305 8564_ $$uhttps://infoscience.epfl.ch/record/125305/files/Villiger_M.pdf$$zn/a$$s1201984
000125305 909C0 $$xU11257$$0252103$$pUPBRI
000125305 909CO $$qGLOBAL_SET$$pconf$$ooai:infoscience.tind.io:125305$$pSV
000125305 937__ $$aUPBRI-CONF-2008-001
000125305 973__ $$rNON-REVIEWED$$sPUBLISHED$$aEPFL
000125305 980__ $$aCONF