Nucleotide detection by fluorescence resonance energy transfer and methods thereof

The present invention provides novel methods for detecting the incorporation of a nucleotide after a single base extension reaction. The methods make use of the Fluorescence Resonance Energy Transfer (FRET) effect generated by two fluorophores linked to the same nucleic acid strand. The methods can be used, either in solution or a solid support, for applications requiring the rapid and precise identification of a nucleotide in a specific position of a nucleic acid, as for genotyping studies


Year:
2003
Note:
Alternative title(s) : (fr) Detection de nucleotides par transfert d'energie par resonance de fluorescence et procedes correspondants
Other identifiers:
EPO Family ID: 27675737
Laboratories:




 Record created 2008-05-13, last modified 2019-12-05


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