Preparation of templates for nucleic acid sequencing

The invention relates to methods of generating templates for a nucleic acid sequencing reaction which comprise: providing at least one double-stranded nucleic acid molecule, wherein both strands of the double-stranded nucleic acid molecule are attached to a solid support at the 5' end, cleaving one or both strands of the double-stranded nucleic acid molecule, and subjecting the cleaved strand(s) to denaturing conditions to remove the portion of the cleaved strand(s) not attached to the solid support, thereby generating a partially or substantially single-stranded template for a nucleic acid sequencing reaction


Year:
2009
Note:
Also published as: WO2007010251 / WO2007010251 / EP1910559
Other identifiers:
EPO Family ID: 34897551
Laboratories:




 Record created 2008-05-13, last modified 2018-03-17


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