Infoscience

Presentation / Talk

Dual-color Total Internal Reflection Fluorescence Fluctuation Spectroscopy

We developed a dual-color total internal reflection fluorescence (TIRF) system for single molecule imaging and fluorescence fluctuation spectroscopy (FFS). For a performance analysis, we measured a synthetic binding assay with dual-color global fluorescence correlation spectroscopy (2CG-FCS) and dual-color fluorescence intensity distribution analysis (2D-FIDA). We show that dual-color TIR-FFS is a suitable method for measuring coincidence assays at the surface. In particular, our setup has a very high fluorescence collection efficiency resulting in a two- to three-fold increased molecular brightness. Using a customized workbench setup, we improved the setup alignment, the sample handling and the system versatility.

    Reference

    • LOB-PRESENTATION-2008-003

    Record created on 2008-02-27, modified on 2016-08-08

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