000117821 001__ 117821
000117821 005__ 20180317093947.0
000117821 0247_ $$2doi$$a10.1093/cercor/bhh092
000117821 02470 $$2ISI$$a000225077400003
000117821 02470 $$2PMID$$a15192011
000117821 037__ $$aARTICLE
000117821 041__ $$aeng
000117821 245__ $$aCorrelation maps allow neuronal electrical properties to be predicted from single-cell gene expression profiles in rat neocortex
000117821 269__ $$a2004
000117821 260__ $$c2004
000117821 336__ $$aJournal Articles
000117821 500__ $$aBrain and Mind Institute, EPFL, Lausanne 1015, Switzerland.
000117821 520__ $$aThe computational power of the neocortex arises from interactions of multiple neurons, which display a wide range of electrical properties. The gene expression profiles underlying this phenotypic diversity are unknown. To explore this relationship, we combined whole-cell electrical recordings with single-cell multiplex RT-PCR of rat (p13-16) neocortical neurons to obtain cDNA libraries of 26 ion channels (including voltage activated potassium channels, Kv1.1/2/4/6, Kvbeta1/2, Kv2.1/2, Kv3.1/2/3/4, Kv4.2/3; sodium/potassium permeable hyperpolarization activated channels, HCN1/2/3/4; the calcium activated potassium channel, SK2; voltage activated calcium channels, Caalpha1A/B/G/I, Cabeta1/3/4), three calcium binding proteins (calbindin, parvalbumin and calretinin) and GAPDH. We found a previously unreported clustering of ion channel genes around the three calcium-binding proteins. We further determined that cells similar in their expression patterns were also similar in their electrical properties. Subsequent regression modeling with statistical resampling yielded a set of coefficients that reliably predicted electrical properties from the expression profile of individual neurons. This is the first report of a consistent relationship between the co-expression of a large profile of ion channel and calcium binding protein genes and the electrical phenotype of individual neocortical neurons.
000117821 700__ $$aToledo-Rodriguez, M.
000117821 700__ $$aBlumenfeld, B.
000117821 700__ $$aWu, C.
000117821 700__ $$aLuo, J.
000117821 700__ $$aAttali, B.
000117821 700__ $$aGoodman, P.
000117821 700__ $$0240392$$aMarkram, H.$$g150822
000117821 773__ $$j14$$k12$$q1310-27$$tCereb Cortex
000117821 909CO $$ooai:infoscience.tind.io:117821$$particle$$pSV
000117821 909C0 $$0252120$$pLNMC$$xU10458
000117821 937__ $$aLNMC-ARTICLE-2004-006
000117821 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000117821 980__ $$aARTICLE