000117721 001__ 117721
000117721 005__ 20181203021112.0
000117721 0247_ $$2doi$$a10.1016/S0092-8674(03)00310-6
000117721 037__ $$aARTICLE
000117721 245__ $$aA global control region defines a chromosomal regulatory landscape containing the HoxD cluster
000117721 269__ $$a2003
000117721 260__ $$c2003
000117721 336__ $$aJournal Articles
000117721 500__ $$aDepartment of Zoology and Animal Biology, NCCR Frontiers in Genetics, University of Geneva, Sciences III, Quai Ernest Ansermet 30, 1211 Geneva 4, Switzerland.
000117721 520__ $$aDuring limb development, coordinated expression of several Hoxd genes is required in presumptive digits. We searched for the underlying control sequences upstream from the cluster and found Lunapark (Lnp), a gene which shares limb and CNS expression specificities with both Hoxd genes and Evx2, another gene located nearby. We used a targeted enhancer-trap approach to identify a DNA segment capable of directing reporter gene expression in both digits and CNS, following Lnp, Evx2, and Hoxd-specific patterns. This DNA region showed an unusual interspecies conservation, including with its pufferfish counterpart. It contains a cluster of global enhancers capable of controlling transcription of several genes unrelated in structure or function, thus defining large regulatory domains. These domains were interrupted in the Ulnaless mutation, a balanced inversion that modified the topography of the locus. We discuss the heuristic value of these results in term of locus specific versus gene-specific regulation.
000117721 700__ $$aSpitz, F.
000117721 700__ $$aGonzalez, F.
000117721 700__ $$g141236$$aDuboule, D.$$0240276
000117721 773__ $$j113$$tCell$$k3$$q405-17
000117721 909C0 $$xU11705$$0252099$$pUPDUB
000117721 909CO $$pSV$$particle$$ooai:infoscience.tind.io:117721
000117721 937__ $$aUPDUB-ARTICLE-2003-003
000117721 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000117721 980__ $$aARTICLE