000117662 001__ 117662
000117662 005__ 20190527152048.0
000117662 037__ $$aARTICLE
000117662 245__ $$aStructure and activity of regulatory elements involved in the activation of the Hoxd-11 gene during late gastrulation
000117662 269__ $$a1993
000117662 260__ $$c1993
000117662 336__ $$aJournal Articles
000117662 500__ $$aEuropean Molecular Biology Laboratory, Heidelberg, Germany.
000117662 520__ $$aWe have used reporter gene constructs to study the cis regulation of the Hoxd-11 gene (previously Hox-4.6) in transgenic mice. We identified a 5 kb regulatory unit, which was able to reproduce important aspects of the initial activation of the gene along the major body axis. The comparison of the nucleotide sequence of this DNA fragment with the corresponding avian genomic region revealed the presence of seven highly homologous stretches of DNA outside the protein coding regions. In particular, the 3' flanking region contained two such domains that are required to mediate the embryonic activation. A chimeric construct containing the two short homologous regions from the chicken gene could replace the complete murine fragment thus demonstrating that the conserved domains carry the main regulatory elements involved in this activation. The first half of this bipartite regulatory region has enhancer activity when tested with a heterologous promoter, while the second half is required to restrict the enhancer activity to the proper expression domain. These results suggest that stage- and tissue-specific cooperation between regulatory elements is required to control properly the activity of the Hoxd-11 promoter.
000117662 700__ $$aGerard, M.
000117662 700__ $$0240276$$g141236$$aDuboule, D.
000117662 700__ $$aZakany, J.
000117662 773__ $$j12$$tEMBO Journal$$k9$$q3539-50
000117662 909C0 $$xU11705$$0252099$$pUPDUB
000117662 909CO $$pSV$$particle$$ooai:infoscience.tind.io:117662
000117662 937__ $$aUPDUB-ARTICLE-1993-004
000117662 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000117662 980__ $$aARTICLE