Dynamic disorder in horseradish peroxidase observed with total internal reflection fluorescence correlation spectroscopy

This paper discusses the application of objective-type total internal reflection fluorescence correlation spectroscopy (TIR-FCS) to the study of the kinetics of immobilized horseradish peroxidase on a single molecule level. Objective-type TIR-FCS combines the advantages of FCS with TIRF microscopy in a way that allows for simultaneous ultra-sensitive spectroscopic measurements using a single-point detector and convenient localization of single molecules on a surface by means of parallel imaging.


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Optics Express, 15, 9, 5366-5375
Year:
2007
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 Record created 2008-02-25, last modified 2018-03-17

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