000117580 001__ 117580
000117580 005__ 20181203021109.0
000117580 037__ $$aARTICLE
000117580 245__ $$aDirect interaction of nucleoporin p62 with mRNA during its export from the nucleus
000117580 269__ $$a1995
000117580 260__ $$c1995
000117580 336__ $$aJournal Articles
000117580 500__ $$aSwiss Institute for Experimental Cancer Research (ISREC) Genetics Unit, Epalinges.
000117580 520__ $$aPrimary transcripts in eukaryotic cells undergo several processing steps within the nucleus, and resulting mature RNA molecules are selectively exported to the cytoplasm. Nucleo-cytoplasmic mRNA transport is an active process that likely involves RNA-protein interactions. To identify specific RNA-binding proteins, we designed a novel approach, which allows the analysis of interactions between mRNAs and proteins along the transport pathway. The method consists of inducing in vivo a covalent binding between nuclear proteins and microinjected mRNAs. Using such a procedure, we were able to detect a direct interaction between nucleoporin p62 with mRNA during export. The formation of the mRNA-p62 complex was inhibited by wheat-germ agglutinin, an inhibitor of mRNA export. Antibodies directed against p62 caused a substantial reduction in the rate of mRNA export from the nucleus.
000117580 700__ $$aDargemont, C.
000117580 700__ $$aSchmidt-Zachmann, M. S.
000117580 700__ $$0243608$$aKühn, L. C.$$g169558
000117580 773__ $$j108 ( Pt 1)$$q257-63$$tJ Cell Sci
000117580 909C0 $$0252245$$pGR-KUHN$$xU11781
000117580 909CO $$ooai:infoscience.tind.io:117580$$pSV$$particle
000117580 937__ $$aGR-KUHN-ARTICLE-1995-001
000117580 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000117580 980__ $$aARTICLE