Analysis of the mouse Scnn1a promoter in cortical collecting duct cells and in transgenic mice
We have isolated and characterised the promoter of the mouse Scnn1a (alpha ENaC) gene. Using transient transfections of serial deletion mutants into Scnn1a-expressing cells, we demonstrate that 1.56 kb of 5' upstream sequence is required for cell-specific expression and corticosteroid-mediated regulation. These 5' sequences are not sufficient to drive expression of a lacZ reporter gene or a rat Scnn1a cDNA in transgenic mice, where they failed to rescue Scnn1a deficiency.
Institute of Pharmacology and Toxicology, University of Lausanne, Switzerland.
Record created on 2008-01-10, modified on 2016-08-08