Analysis of the mouse Scnn1a promoter in cortical collecting duct cells and in transgenic mice

We have isolated and characterised the promoter of the mouse Scnn1a (alpha ENaC) gene. Using transient transfections of serial deletion mutants into Scnn1a-expressing cells, we demonstrate that 1.56 kb of 5' upstream sequence is required for cell-specific expression and corticosteroid-mediated regulation. These 5' sequences are not sufficient to drive expression of a lacZ reporter gene or a rat Scnn1a cDNA in transgenic mice, where they failed to rescue Scnn1a deficiency.


Published in:
Biochim Biophys Acta, 1519, 1-2, 106-10
Year:
2001
Note:
Institute of Pharmacology and Toxicology, University of Lausanne, Switzerland.
Laboratories:




 Record created 2008-01-10, last modified 2018-03-17


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