Single-channel recordings of volume-sensitive Cl- channels in human intestinal epithelial cells

Under the double-patch configuration, stepwise closing unitary events could be observed in the on-cell patch upon applications of large positive potentials only when inactivating whole-cell Cl- currents were simultaneously observed in cultured human epithelial cells (Intestine 407) after osmotic swelling. Under the cell-attached configuration, the single channel events exhibited similar time- and voltage-dependent inactivation upon large depolarizations. The inactivation time course became shorter with increasing positive command pulses. The unitary slope conductance was around 63 pS at +140 mV and 46 pS at +100 mV exhibiting outward rectification.


Published in:
Jpn J Physiol, 44 Suppl 2, S73-5
Year:
1994
Note:
Department of Cellular and Molecular Physiology, National Institute for Physiological Sciences, Okazaki, Japan.
Laboratories:




 Record created 2007-12-18, last modified 2018-03-17


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