000114911 001__ 114911
000114911 005__ 20181203021033.0
000114911 0247_ $$2doi$$a10.1186/1471-2164-7-176
000114911 02470 $$2DAR$$a9370
000114911 02470 $$2ISI$$a000240731100001
000114911 037__ $$aARTICLE
000114911 245__ $$aSimilarities and differences of polyadenylation signals in human and fly
000114911 269__ $$a2006
000114911 260__ $$c2006
000114911 336__ $$aJournal Articles
000114911 520__ $$aABSTRACT: BACKGROUND: Cleavage of messenger RNA (mRNA) precursors is an essential step in mRNA maturation. The signal recognized by the cleavage enzyme complex has been characterized as an A rich region upstream of the cleavage site containing a motif with consensus AAUAAA, followed by a U or UG rich region downstream of the cleavage site. RESULTS: We studied these signals using exhaustive databases of cleavage sites obtained from aligning raw expressed sequence tags (EST) sequences to genomic sequences in Homo sapiens and Drosophila melanogaster. These data show that the polyadenylation signal is highly conserved in human and fly. In addition, de novo motif searches generated a refined description of the U-rich downstream sequence (DSE) element, which shows more divergence between the two species. These refined motifs are applied, within a Hidden Markov Model (HMM) framework, to predict mRNA cleavage sites. CONCLUSIONS: We demonstrate that the DSE is a specific motif in both human and Drosophila. These findings shed light on the sequence correlates of a highly conserved biological process, and improve in silico prediction of 3P mRNA cleavage and polyadenylation sites.
000114911 700__ $$aRetelska, D.
000114911 700__ $$aIseli, C.
000114911 700__ $$0244404$$aBucher, P.$$g113607
000114911 700__ $$aJongeneel, C. V.
000114911 700__ $$0240296$$aNaef, F.$$g110606
000114911 773__ $$j7$$k1$$q176$$tBMC Genomics
000114911 909C0 $$0252244$$pGR-BUCHER$$xU11780
000114911 909C0 $$0252319$$pUPNAE$$xU11260
000114911 909CO $$ooai:infoscience.tind.io:114911$$pSV$$particle
000114911 937__ $$aGR-BUCHER-ARTICLE-2006-004
000114911 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000114911 980__ $$aARTICLE