000114900 001__ 114900
000114900 005__ 20190527125158.0
000114900 037__ $$aARTICLE
000114900 245__ $$aLong distance control of MHC class II expression by multiple distal enhancers regulated by regulatory factor X complex and CIITA
000114900 269__ $$a2004
000114900 260__ $$c2004
000114900 336__ $$aJournal Articles
000114900 500__ $$aUniversity of Geneva Medical School, Centre Medical Universitaire, 1 rue Michel-Servet, CH-1211 Geneva, Switzerland.
000114900 520__ $$aMHC class II (MHC-II) genes are regulated by an enhanceosome complex containing two gene-specific transcription factors, regulatory factor X complex (RFX) and CIITA. These factors assemble on a strictly conserved regulatory module (S-X-X2-Y) found immediately upstream of the promoters of all classical and nonclassical MHC-II genes as well as the invariant chain (Ii) gene. To identify new targets of RFX and CIITA, we developed a computational approach based on the unique and highly constrained architecture of the composite S-Y motif. We identified six novel S'-Y' modules situated far away from the promoters of known human RFX- and CIITA-controlled genes. Four are situated at strategic positions within the MHC-II locus, and two are found within the Ii gene. These S'-Y' modules function as transcriptional enhancers, are bona fide targets of RFX and CIITA in B cells and IFN-gamma-induced cells, and induce broad domains of histone hyperacetylation. These results reveal a hitherto unexpected level of complexity involving long distance control of MHC-II expression by multiple distal regulatory elements.
000114900 700__ $$aKrawczyk, M.
000114900 700__ $$aPeyraud, N.
000114900 700__ $$aRybtsova, N.
000114900 700__ $$aMasternak, K.
000114900 700__ $$0244404$$g113607$$aBucher, P.
000114900 700__ $$aBarras, E.
000114900 700__ $$aReith, W.
000114900 773__ $$j173$$tJournal of Immunology$$k10$$q6200-10
000114900 909C0 $$xU11780$$0252244$$pGR-BUCHER
000114900 909CO $$pSV$$particle$$ooai:infoscience.tind.io:114900
000114900 937__ $$aGR-BUCHER-ARTICLE-2004-002
000114900 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000114900 980__ $$aARTICLE