000114895 001__ 114895
000114895 005__ 20181203021032.0
000114895 037__ $$aARTICLE
000114895 245__ $$aClassification of human astrocytic gliomas on the basis of gene expression: a correlated group of genes with angiogenic activity emerges as a strong predictor of subtypes
000114895 269__ $$a2003
000114895 260__ $$c2003
000114895 336__ $$aJournal Articles
000114895 500__ $$aLaboratory of Tumor Biology and Genetics,University Hospital (CHUV), 1011 Lausanne, Switzerland.
000114895 520__ $$aThe development of targeted treatment strategies adapted to individual patients requires identification of the different tumor classes according to their biology and prognosis. We focus here on the molecular aspects underlying these differences, in terms of sets of genes that control pathogenesis of the different subtypes of astrocytic glioma. By performing cDNA-array analysis of 53 patient biopsies, comprising low-grade astrocytoma, secondary glioblastoma (respective recurrent high-grade tumors), and newly diagnosed primary glioblastoma, we demonstrate that human gliomas can be differentiated according to their gene expression. We found that low-grade astrocytoma have the most specific and similar expression profiles, whereas primary glioblastoma exhibit much larger variation between tumors. Secondary glioblastoma display features of both other groups. We identified several sets of genes with relatively highly correlated expression within groups that: (a). can be associated with specific biological functions; and (b). effectively differentiate tumor class. One prominent gene cluster discriminating primary versus nonprimary glioblastoma comprises mostly genes involved in angiogenesis, including VEGF fms-related tyrosine kinase 1 but also IGFBP2, that has not yet been directly linked to angiogenesis. In situ hybridization demonstrating coexpression of IGFBP2 and VEGF in pseudopalisading cells surrounding tumor necrosis provided further evidence for a possible involvement of IGFBP2 in angiogenesis. The separating groups of genes were found by the unsupervised coupled two-way clustering method, and their classification power was validated by a supervised construction of a nearly perfect glioma classifier.
000114895 700__ $$aGodard, S.
000114895 700__ $$aGetz, G.
000114895 700__ $$aDelorenzi, M.
000114895 700__ $$aFarmer, P.
000114895 700__ $$aKobayashi, H.
000114895 700__ $$aDesbaillets, I.
000114895 700__ $$aNozaki, M.
000114895 700__ $$aDiserens, A. C.
000114895 700__ $$aHamou, M. F.
000114895 700__ $$aDietrich, P. Y.
000114895 700__ $$aRegli, L.
000114895 700__ $$aJanzer, R. C.
000114895 700__ $$0244404$$g113607$$aBucher, P.
000114895 700__ $$aStupp, R.
000114895 700__ $$ade Tribolet, N.
000114895 700__ $$aDomany, E.
000114895 700__ $$aHegi, M. E.
000114895 773__ $$j63$$tCancer Res$$k20$$q6613-25
000114895 909C0 $$xU11780$$0252244$$pGR-BUCHER
000114895 909CO $$pSV$$particle$$ooai:infoscience.tind.io:114895
000114895 937__ $$aGR-BUCHER-ARTICLE-2003-003
000114895 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000114895 980__ $$aARTICLE