000114883 001__ 114883
000114883 005__ 20181203021032.0
000114883 0247_ $$2doi$$a10.1385/IR:23:1:75
000114883 037__ $$aARTICLE
000114883 245__ $$aIndividuality of Ag-selected and preimmune TCR repertoires
000114883 269__ $$a2001
000114883 260__ $$c2001
000114883 336__ $$aJournal Articles
000114883 500__ $$aINSERM Unit 503, Lyon, France. maryanski@cervi-lyon.inserm.fr
000114883 520__ $$aWe have combined flow cytometry and single-cell PCR to characterize the TCRBV repertoires selected by individual mice in a model CD8 response against a defined peptide/MHC complex (CW3 170-1 79/Kd). Ourresults established thatdifferent mice select individually distinct yet structurally similar CW3-specific repertoires. Repertoire selection appears to be flexible depending on the immunizing cell dose. Using a single-donor, matched-pair-recipient adoptive transfer strategy, we demonstrated that the CW3-specific TCR repertoires of normal mice are already distinct at the preimmune level. We combine our data with computer simulations to test models for the composition of an Ag-specific preimmune repertoire and its selection during an immune response.
000114883 700__ $$aMaryanski, J. L.
000114883 700__ $$aAttuil, V.
000114883 700__ $$aHamrouni, A.
000114883 700__ $$aMutin, M.
000114883 700__ $$aRossi, M.
000114883 700__ $$aAublin, A.
000114883 700__ $$0244404$$aBucher, P.$$g113607
000114883 773__ $$j23$$k1$$q75-84$$tImmunol Res
000114883 909C0 $$0252244$$pGR-BUCHER$$xU11780
000114883 909CO $$ooai:infoscience.tind.io:114883$$pSV$$particle
000114883 937__ $$aGR-BUCHER-ARTICLE-2001-005
000114883 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000114883 980__ $$aARTICLE