000114876 001__ 114876
000114876 005__ 20181203021032.0
000114876 0247_ $$2doi$$a10.1073/pnas.97.7.3491
000114876 037__ $$aARTICLE
000114876 245__ $$aShotgun sequencing of the human transcriptome with ORF expressed sequence tags
000114876 269__ $$a2000
000114876 260__ $$c2000
000114876 336__ $$aJournal Articles
000114876 500__ $$aLudwig Institute for Cancer Research, Sao Paulo 01509-010, Brazil.
000114876 520__ $$aTheoretical considerations predict that amplification of expressed gene transcripts by reverse transcription-PCR using arbitrarily chosen primers will result in the preferential amplification of the central portion of the transcript. Systematic, high-throughput sequencing of such products would result in an expressed sequence tag (EST) database consisting of central, generally coding regions of expressed genes. Such a database would add significant value to existing public EST databases, which consist mostly of sequences derived from the extremities of cDNAs, and facilitate the construction of contigs of transcript sequences. We tested our predictions, creating a database of 10,000 sequences from human breast tumors. The data confirmed the central distribution of the sequences, the significant normalization of the sequence population, the frequent extension of contigs composed of existing human ESTs, and the identification of a series of potentially important homologues of known genes. This approach should make a significant contribution to the early identification of important human genes, the deciphering of the draft human genome sequence currently being compiled, and the shotgun sequencing of the human transcriptome.
000114876 700__ $$aDias Neto, E.
000114876 700__ $$aCorrea, R. G.
000114876 700__ $$aVerjovski-Almeida, S.
000114876 700__ $$aBriones, M. R.
000114876 700__ $$aNagai, M. A.
000114876 700__ $$ada Silva, W. Jr.
000114876 700__ $$aZago, M. A.
000114876 700__ $$aBordin, S.
000114876 700__ $$aCosta, F. F.
000114876 700__ $$aGoldman, G. H.
000114876 700__ $$aCarvalho, A. F.
000114876 700__ $$aMatsukuma, A.
000114876 700__ $$aBaia, G. S.
000114876 700__ $$aSimpson, D. H.
000114876 700__ $$aBrunstein, A.
000114876 700__ $$ade Oliveira, P. S.
000114876 700__ $$0244404$$g113607$$aBucher, P.
000114876 700__ $$aJongeneel, C. V.
000114876 700__ $$aO'Hare, M. J.
000114876 700__ $$aSoares, F.
000114876 700__ $$aBrentani, R. R.
000114876 700__ $$aReis, L. F.
000114876 700__ $$ade Souza, S. J.
000114876 700__ $$aSimpson, A. J.
000114876 773__ $$j97$$tProc Natl Acad Sci U S A$$k7$$q3491-6
000114876 909C0 $$xU11780$$0252244$$pGR-BUCHER
000114876 909CO $$pSV$$particle$$ooai:infoscience.tind.io:114876
000114876 937__ $$aGR-BUCHER-ARTICLE-2000-003
000114876 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000114876 980__ $$aARTICLE