000114861 001__ 114861
000114861 005__ 20181203021031.0
000114861 037__ $$aARTICLE
000114861 245__ $$aThe discoidin domain family revisited: new members from prokaryotes and a homology-based fold prediction
000114861 269__ $$a1998
000114861 260__ $$c1998
000114861 336__ $$aJournal Articles
000114861 500__ $$aLund University, Department of Cell & Molecular Biology, Sweden. Stefan.Baumgartner@medkem.lu.se
000114861 520__ $$aMembers of the discoidin (DS) domain family, which includes the C1 and C2 repeats of blood coagulation factors V and VIII, occur in a great variety of eukaryotic proteins, most of which have been implicated in cell-adhesion or developmental processes. So far, no three-dimensional structure of a known example of this extracellular module has been determined, limiting the usefulness of identifying a new sequence as member of this family. Here, we present results of a recent search of the protein sequence database for new DS domains using generalized profiles, a sensitive multiple alignment-based search technique. Several previously unrecognized DS domains could be identified by this method, including the first examples from prokaryotic species. More importantly, we present statistical, structural, and functional evidence that the D1 domain of galactose oxidase whose three-dimensional structure has been determined at 1.7 A resolution, is a distant member of this family. Taken together, these findings significantly expand the concept of the DS domain, by extending its taxonomic range and by implying a fold prediction for all its members. The proposed alignment with the galactose oxidase sequence makes it possible to construct homology-based three-dimensional models for the most interesting examples, as illustrated by an accompanying paper on the C1 and C2 domains of factor V.
000114861 700__ $$aBaumgartner, S.
000114861 700__ $$aHofmann, K.
000114861 700__ $$aChiquet-Ehrismann, R.
000114861 700__ $$g113607$$aBucher, P.$$0244404
000114861 773__ $$j7$$tProtein Sci$$k7$$q1626-31
000114861 909C0 $$xU11780$$0252244$$pGR-BUCHER
000114861 909CO $$pSV$$particle$$ooai:infoscience.tind.io:114861
000114861 937__ $$aGR-BUCHER-ARTICLE-1998-001
000114861 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000114861 980__ $$aARTICLE