000114855 001__ 114855
000114855 005__ 20181203021031.0
000114855 0247_ $$2doi$$a10.1093/intimm/8.7.1131
000114855 037__ $$aARTICLE
000114855 245__ $$aMemory TCR repertoires analyzed long-term reflect those selected during the primary response
000114855 269__ $$a1996
000114855 260__ $$c1996
000114855 336__ $$aJournal Articles
000114855 500__ $$aLudwig Institue for Cancer Research, Lausanne Branch, University of Lausanne, Switzerland.
000114855 520__ $$aNormal T cell repertoire selection and evolution in antigen-specific responses is poorly understood. We have recently described an MHC class I-restricted response characterized by an overwhelming expansion of CD8 cells expressing a Vbeta10 TCR, thus allowing the identification of antigen-selected cells directly ex vivo. Our present strategy to follow the overall TCR repertoire selection was to monitor the expression of a particular TCR alpha chain (Valpha8) on antigen-selected Vbeta10(+) cells by four-color flow cytometry. We demonstrate that while there is substantial variation among the responder mice in Valpha8 usage, the repertoires of individual animals remain relatively stable over long periods of time (>1 year), with or without repeated antigenic challenge. Thus if any evolution of this response occurs upon re-exposure to antigen, it would appear not to skew the TCR repertoire established during the primary response.
000114855 700__ $$aWalker, P. R.
000114855 700__ $$aWilson, A.
000114855 700__ $$0244404$$aBucher, P.$$g113607
000114855 700__ $$aMaryanski, J. L.
000114855 773__ $$j8$$k7$$q1131-8$$tInt Immunol
000114855 909C0 $$0252244$$pGR-BUCHER$$xU11780
000114855 909CO $$ooai:infoscience.tind.io:114855$$pSV$$particle
000114855 937__ $$aGR-BUCHER-ARTICLE-1996-008
000114855 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000114855 980__ $$aARTICLE