000114854 001__ 114854
000114854 005__ 20181203021031.0
000114854 0247_ $$2doi$$a10.1016/S1074-7613(00)80297-6
000114854 037__ $$aARTICLE
000114854 245__ $$aSingle-cell PCR analysis of TCR repertoires selected by antigen in vivo: a high magnitude CD8 response is comprised of very few clones
000114854 269__ $$a1996
000114854 260__ $$c1996
000114854 336__ $$aJournal Articles
000114854 500__ $$aLudwig Institute for Cancer Research, Swiss Institute for Experimental Cancer Research, Epalinges, Switzerland.
000114854 520__ $$aTaking advantage of a potent MHC class I-restricted response that allows the identification of antigen-selected CD8 T cells directly ex vivo, we characterized the antigen-specific T cell repertoires that develop in individual mice by single-cell PCR analysis. Each of the immune mice displayed distinct yet structurally similar TCR repertoires. The overall repertoire size was estimated to be in the range of 15-20 for most mice. No major differences were observed between primary and secondary responses. Moreover, for a hyperimmunized mouse the antigen-specific TCR repertoire expressed 8 months after the initial immunization was very similar to that found at the peak of the primary response. Our results demonstrate that a high magnitude immune response may be composed of very few clones, and that at least in the system analyzed, the memory response largely reflects the repertoire selected by the peak of the primary response.
000114854 700__ $$aMaryanski, J. L.
000114854 700__ $$aJongeneel, C. V.
000114854 700__ $$0244404$$g113607$$aBucher, P.
000114854 700__ $$aCasanova, J. L.
000114854 700__ $$aWalker, P. R.
000114854 773__ $$j4$$tImmunity$$k1$$q47-55
000114854 909C0 $$xU11780$$0252244$$pGR-BUCHER
000114854 909CO $$pSV$$particle$$ooai:infoscience.tind.io:114854
000114854 937__ $$aGR-BUCHER-ARTICLE-1996-007
000114854 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000114854 980__ $$aARTICLE