000114830 001__ 114830
000114830 005__ 20181203021031.0
000114830 0247_ $$2doi$$a10.1093/nar/14.24.10009
000114830 037__ $$aARTICLE
000114830 245__ $$aCompilation and analysis of eukaryotic POL II promoter sequences
000114830 269__ $$a1986
000114830 260__ $$c1986
000114830 336__ $$aJournal Articles
000114830 520__ $$aA representative set of 168 eukaryotic POL II promoters has been compiled from the EMBL library and subjected to computer signal search analysis. Application of this technique to E. coli promoters as a control ensemble revealed the well known consensus sequences at -35 and -10 which indicates that the methods are adequate to approach problems of this kind. The results obtained from the eukaryotic promoter set can be summarized as follows: Common sequence features are confined to a region between -50 and +10 relative to the transcriptional initiation site. The only well conserved consensus sequence is TATAAA, centered at -28. A weak motif, CA followed preferentially by pyrimidines, surrounds the cap-site. Two pentanucleotides which have been shown by experiments to stimulate transcription of certain genes, GGGCG and CCAAT, are moderately over-represented in the upstream region (between -129 and -50). However, they occur at highly variable distances from the initiation site.
000114830 700__ $$0244404$$g113607$$aBucher, P.
000114830 700__ $$aTrifonov, E. N.
000114830 773__ $$j14$$tNucleic Acids Res$$k24$$q10009-26
000114830 909C0 $$xU11780$$0252244$$pGR-BUCHER
000114830 909CO $$pSV$$particle$$ooai:infoscience.tind.io:114830
000114830 937__ $$aGR-BUCHER-ARTICLE-1986-001
000114830 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000114830 980__ $$aARTICLE