000114828 001__ 114828
000114828 005__ 20181203021030.0
000114828 0247_ $$2doi$$a10.1093/nar/11.24.8641
000114828 037__ $$aARTICLE
000114828 245__ $$aTranscription of a cloned Xenopus laevis H4 histone gene in the homologous frog oocyte system depends on an evolutionary conserved sequence motif in the -50 region
000114828 269__ $$a1983
000114828 260__ $$c1983
000114828 336__ $$aJournal Articles
000114828 520__ $$aA cloned Xenopus laevis H4 histone gene has been expressed in the X.laevis oocyte nucleus. The homologous histone H4 gene can be correctly and efficiently expressed in the frog oocyte even in presence of bacterial vector DNA. As revealed by both analytical gel electrophoresis and S1 mapping, two H4 mRNAs are specified with different transcriptional efficiencies from the tandemly repeated promoter. Results from deletion mapping of the sequences essential for promoting H4 transcription show that drastic reduction of transcription is obtained when the sequences lying between -64 and -35 bp from the mRNA cap site are removed. We demonstrate by DNA sequence comparison using a novel computer program that this important area of the H4 promoter contains two highly conserved DNA motifs near positions -51 to -46 upstream from the cap site in all H4 gene promoters analysed.
000114828 700__ $$aClerc, R. G.
000114828 700__ $$0244404$$g113607$$aBucher, P.
000114828 700__ $$aStrub, K.
000114828 700__ $$aBirnstiel, M. L.
000114828 773__ $$j11$$tNucleic Acids Res$$k24$$q8641-57
000114828 909C0 $$xU11780$$0252244$$pGR-BUCHER
000114828 909CO $$pSV$$particle$$ooai:infoscience.tind.io:114828
000114828 937__ $$aGR-BUCHER-ARTICLE-1983-001
000114828 973__ $$rREVIEWED$$sPUBLISHED$$aOTHER
000114828 980__ $$aARTICLE