000114618 001__ 114618
000114618 005__ 20190316234104.0
000114618 0247_ $$2doi$$a10.1002/chem.200700883
000114618 02470 $$2ISI$$a000251391000009
000114618 02470 $$2DAR$$a11712
000114618 037__ $$aARTICLE
000114618 245__ $$aA Polyoxyethylene-Substituted Bimetallic Europium Helicate for Luminescent Staining of Living Cells
000114618 269__ $$a2007
000114618 260__ $$c2007
000114618 336__ $$aJournal Articles
000114618 520__ $$aThe homoditopic ligand $H_2L^{C3}$ has been designed to form neutral triple-stranded bimetallic helicates of overall composition [$Ln_2(L^{C3})_3$]. The grafting of the polyoxyethylene fragments ensures water solubility and also favors cell penetration while being amenable to further derivatization. The ligand $pK_a$ values have been determined by spectrophotometric titration and range from 3.5 (sum of the first two) to 10.3. The thermodynamic stability of the helicates is large at physiological pH ($\log\beta_{23}$ in the range 22–23). The ligand triplet state has an adequate energy (0–phonon transition at $\approx20 800 cm^{-1}$) for sensitizing the luminescence of $Eu^{III}$ (Q=11%). Analysis of the $Eu^{III}$ emission spectrum points to an overall pseudo $D_3$ symmetry for the metal environment. No significant effect of [$Eu_2(L^ {C3})_3$] is observed on the viability of several cancerous cell lines (MCF-7, HeLa, Jurkat, and 5D10). The cell imaging properties of the $Eu^{III}$ helicate are demonstrated for the HeLa cell line by luminescence microscopy. Bright $Eu^{III}$ emission is seen for helicate concentration $>50 \mu M$ and after 20– 30 min loading time. The helicate stains the cytoplasm and the permeation mechanism is likely to be endocytosis.
000114618 6531_ $$abimetallic compounds
000114618 6531_ $$ahelicates
000114618 6531_ $$aluminescence
000114618 700__ $$0240735$$g149479$$aChauvin, Anne-Sophie
000114618 700__ $$0241079$$g148905$$aComby, Steve
000114618 700__ $$0242690$$g176618$$aSong, Bo
000114618 700__ $$g138412$$aVandevyver, C. D. B.$$0240440
000114618 700__ $$aThomas, Frédéric
000114618 700__ $$aBünzli, Jean-Claude G.$$g122803$$0241990
000114618 773__ $$j13$$tChemistry - A European Journal$$q9515-9526
000114618 8564_ $$zURL
000114618 8564_ $$uhttps://infoscience.epfl.ch/record/114618/files/CEJ-07-Cell_Imaging_LC3.pdf$$zn/a$$s526858
000114618 909C0 $$xU10099$$0252081$$pLCSL
000114618 909CO $$ooai:infoscience.tind.io:114618$$qGLOBAL_SET$$pSB$$particle
000114618 937__ $$aLCSL-ARTICLE-2007-013
000114618 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000114618 980__ $$aARTICLE