We designed a method termed Telospot to discover and characterize telomerase modulators as anticancer drugs or chemical biology tools. Telospot is based on a highly efficient human telomerase expression system and the detection of telomerase DNA reaction products in macroarray format. Telospot offers a highly scalable, cost- and time-effective alternative to presently available telomerase assays, which are limited by the requirement for PCR, telomerase purification or technologies not amenable to high throughput.
Title
Low- to high-throughput analysis of telomerase modulators with Telospot
Published in
Nature Methods
Volume
4
Issue
10
Pages
851-853
Date
2007
Note
[1] Swiss Institute for Experimental Cancer Research, Chemin des Boveresses 155, CH-1066 Epalinges, Switzerland. [2] Ecole Polytechnique Federale de Lausanne, School of Life Sciences, Station 15, CH-1015 Lausanne, Switzerland. [3] "Frontiers in Genetics" National Center for Competence in Research, quai Ernest-Ansermet 30, CH-1211 Geneva, Switzerland. [4] Present address: Institut National de la Sante et de la Recherche Medicale (INSERM), U758, Ecole Normale Superieure de Lyon, 46 allee d'Italie, F-69364 Lyon, France. [5] These authors contributed equally to this work.
Record creation date
2007-11-20
