000114162 001__ 114162
000114162 005__ 20181203021019.0
000114162 0247_ $$2doi$$a10.1038/354496a0
000114162 037__ $$aARTICLE
000114162 245__ $$aCloning and expression of the essential gene for poly(A) polymerase from S. cerevisiae
000114162 269__ $$a1991
000114162 260__ $$c1991
000114162 336__ $$aJournal Articles
000114162 500__ $$aDepartment of Cell Biology, University of Basel, Switzerland.
000114162 520__ $$aPoly(A) polymerase is essential for the maturation of messenger RNA, adding tracts of adenosine residues to the 3' end of precursor RNA generated by endonucleolytic cleavage. This mechanism of mRNA 3' processing seems to be similar in yeast and in higher eucaryotes, although there are differences in the recognition signals in the pre-mRNA. Here we describe the cloning of the gene for yeast poly(A) polymerase. The enzyme is encoded by a single and essential gene located near the centromere on the left arm of chromosome 11. Poly(A) polymerase purified from recombinant Escherichia coli has the same physical and biochemical properties as the yeast enzyme. The yeast poly(A) polymerase shares features of sequence with its mammalian homologue.
000114162 700__ $$0240570$$g168670$$aLingner, J.
000114162 700__ $$aKellermann, J.
000114162 700__ $$aKeller, W.
000114162 773__ $$j354$$tNature$$k6353$$q496-8
000114162 909C0 $$xU11159$$0252147$$pUPLIN
000114162 909CO $$pSV$$particle$$ooai:infoscience.tind.io:114162
000114162 937__ $$aUPLIN-ARTICLE-1991-002
000114162 970__ $$a93/UPLIN
000114162 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000114162 980__ $$aARTICLE