000113687 001__ 113687
000113687 005__ 20180318102624.0
000113687 0247_ $$2doi$$a10.2533/chimia.2006.754
000113687 02470 $$2ISI$$a000242520700006
000113687 037__ $$aARTICLE
000113687 245__ $$aMicro- and nanostructured devices for investigating biomolecular interactions
000113687 269__ $$a2006
000113687 260__ $$c2006
000113687 336__ $$aJournal Articles
000113687 500__ $$aInvited Paper
000113687 520__ $$aCell membrane receptors and ion channels are essential in many different cellular processes. To analyse the activity of membrane proteins in vesicles and biological cells, we fabricated micro- nanostructured chips, enabling the application of electrophysiology and fluorescence-based techniques. A SU-8 biochip was developed for simultaneously micromanipulating and investigating optically and electrically individual vesicles in a microfluidic channel. Lipid vesicles were transported, positioned by electrophoretic movement on a micrometer sized aperture, and fused to form a planar suspended membrane, which is suited to study ion channel activity. Aiming to investigate G protein-coupled receptor signaling pathways in native-like environment, we developed a method for producing well-oriented planar cell membrane sheets on silicon films containing nanoaperture arrays. The accessibility of extracellular and cytosolic surfaces was demonstrated by targeting membrane constituents side-specifically with fluorescent markers. Our approach can be applied for studying membrane proteins from a large variety of cells and cellular organelles using chip-based screening assays.
000113687 6531_ $$aElectrophysiology
000113687 6531_ $$aFluorescence techniques
000113687 6531_ $$aMembrane proteins
000113687 6531_ $$aMicro- nanoapertures
000113687 700__ $$0240611$$aDanelon, C.$$g162064
000113687 700__ $$aJenke, M.J.
000113687 700__ $$0240643$$aSchreiter, C.$$g138900
000113687 700__ $$0240644$$aKim, G.M.$$g149003
000113687 700__ $$0240994$$aPerez, J.-B.$$g103031
000113687 700__ $$0240847$$aSantschi, C.$$g117095
000113687 700__ $$0240120$$aBrugger, J.$$g145781
000113687 700__ $$0240645$$aVogel, H.$$g106666
000113687 773__ $$j60$$k11$$q754-760$$tCHIMIA
000113687 8564_ $$s6149129$$uhttps://infoscience.epfl.ch/record/113687/files/Chimia_2006.pdf$$zn/a
000113687 909CO $$ooai:infoscience.tind.io:113687$$pSB$$pSTI$$particle
000113687 909C0 $$0252153$$pLCPPM$$xU10170
000113687 909C0 $$0252040$$pLMIS1$$xU10321
000113687 937__ $$aLCPPM-ARTICLE-2007-007
000113687 937__ $$aLMIS1-ARTICLE-2007-003
000113687 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000113687 980__ $$aARTICLE