Bead-based protein microarrays realized through electrostatic self-assembly of carboxylated beads

We report here a simple method, based on electrostatic self-assembly, for realizing bead-based single protein microarrays. We generate positively-charged surface micro-patterns on a glass substrate and used them as the template for adsorbing negatively-charged carboxylated beads. The, positively-charged, template for bead adsorption is generated by patterning a aminosilane layer using conventional lift-off technique. The –COOH groups which are present on the patterned beads’ surface is activated using carbodiimide chemistry and then a protein of interest is covalently coupled to the bead surface. The patterned beads bind strongly to the aminosilane patterns and they are able to even withstand agitated rinse in de-ionized water. We also show here that the same method can be extended for bead patterning with in sealed microfluidic channels.

Published in:
Book of abstracts of MNE 07, 281-282
Presented at:
International conference on Micro- and Nano-Engineering (MNE), Copenhagen, DK, September 24-26 2007

 Record created 2007-09-27, last modified 2018-03-17

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