Durand, N. F. Y.
Bertsch, Arnaud
Todorova, Mina
Renaud, Philippe
Direct Measurement of Effective Diffusion Coefficients in Nanochannels Using Steady-State Dispersion Effects
Applied Physics Letters
Applied Physics Letters
Applied Physics Letters
Applied Physics Letters
91
biodiffusion
microchannel flow
molecular biophysics
proteins
2007
2007
We present a method to measure effective diffusion coefficients of fluorescently labeled molecules inside a nanofluidic system. Molecules with small diffusion coefficients show a larger lateral dispersion than highly diffusive species, which is counterintuitive. We performed measurements with wheat germ agglutinin proteins and obtained an effective diffusion coefficient which is four orders of magnitude lower than its free diffusion coefficient. Our technique which is a direct and relatively simple measurement of the effective diffusion coefficients inside nanochannels of well controlled dimensions could help fundamental studies in membranes and separation sciences.
American Institute of Physics
0003-6951
Applied Physics Letters
Journal Articles
10.1063/1.2801625