Direct Measurement of Effective Diffusion Coefficients in Nanochannels Using Steady-State Dispersion Effects

We present a method to measure effective diffusion coefficients of fluorescently labeled molecules inside a nanofluidic system. Molecules with small diffusion coefficients show a larger lateral dispersion than highly diffusive species, which is counterintuitive. We performed measurements with wheat germ agglutinin proteins and obtained an effective diffusion coefficient which is four orders of magnitude lower than its free diffusion coefficient. Our technique which is a direct and relatively simple measurement of the effective diffusion coefficients inside nanochannels of well controlled dimensions could help fundamental studies in membranes and separation sciences.


Published in:
Applied Physics Letters, 91, 203106
Year:
2007
Publisher:
American Institute of Physics
ISSN:
0003-6951
Keywords:
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 Record created 2007-09-26, last modified 2018-03-17

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