000109606 001__ 109606
000109606 005__ 20190806111823.0
000109606 0247_ $$2doi$$a10.1117/1.1332774
000109606 022__ $$a1083-3668
000109606 02470 $$2DAR$$a1159
000109606 02470 $$2ISI$$a000166802700005
000109606 037__ $$aARTICLE
000109606 245__ $$aIn vivo autofluorescence spectroscopy of human bronchial tissue to optimize the detection and imaging of early cancers
000109606 260__ $$c2001
000109606 269__ $$a2001
000109606 336__ $$aJournal Articles
000109606 520__ $$aWe are developing an imaging system to detect pre-/early cancers in the tracheo-bronchial tree. Autofluorescence might be useful but many features remain suboptimal. We have studied the autofluorescence of human healthy, metaplastic and dysplastic/CIS bronchial tissue, covering excitation wavelengths from 350 to 480 nm. These measurements are performed with a spectrofluorometer whose distal end is designed to simulate the spectroscopic response of an imaging system using routine bronchoscopes. Our data provide information about the excitation and emission spectral ranges to be used in a dual range detection imaging system to maximize the tumor vs healthy and the tumor vs. inflammatory/metaplastic contrast in detecting pre-/early malignant lesions. We find that the excitation wavelengths yielding the highest contrasts are between 400 and 480 nm with a peak at 405 nm. We also find that the shape of the spectra of healthy tissue is similar to that of its inflammatory/metaplastic counterpart. Finally we find that, when the spectra are normalized, the region of divergence between the tumor and the nontumor spectra is consistently between 600 and 800 nm and that the transition wavelength between the two spectral regions is around 590 nm for all the spectra regardless of the excitation wavelength, thus suggesting that there might be one absorber or one fluorophore. The use of backscattered red light enhances the autofluorescence contrast.
000109606 6531_ $$aSpectrometry
000109606 6531_ $$aFluorescence
000109606 700__ $$0246077$$g110512$$aZellweger, M.
000109606 700__ $$aGrosjean, P.
000109606 700__ $$aGoujon, D.
000109606 700__ $$aMonnier, P.
000109606 700__ $$0240886$$g106613$$avan den Bergh, H.
000109606 700__ $$0241203$$g106697$$aWagnières, G.
000109606 773__ $$j6$$tJournal of biomedical optics$$k1$$q41-51
000109606 909CO $$particle$$pSTI$$pSB$$ooai:infoscience.tind.io:109606
000109606 909C0 $$pGR-VDB$$0252359
000109606 909C0 $$0252376$$xU10271$$pLPAS
000109606 909C0 $$pGPM$$0252512$$xS14027
000109606 917Z8 $$x171247
000109606 917Z8 $$x171848
000109606 937__ $$aLPAS-ARTICLE-2001-001
000109606 970__ $$a40/LPAS
000109606 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000109606 980__ $$aARTICLE