Infoscience

Journal article

Time-resolved spectrofluorometer for clinical tissue characterization during endoscopy

Time-resolved fluorescence spectroscopy has the potential to provide more information for the detection of early cancer than continuous wave spectroscopy. A new optical fiber-based spectrofluorometer for time-resolved fluorescence spectroscopy of biol. tissue during clin. endoscopy is presented. The app. is based on a nitrogen laser pumping a dye laser as excitation source and a streak camera coupled with a spectrograph as time-resolved spectrometer. The excitation and fluorescence light is carried by an optical fiber to the tissue under investigation and back to the detector, resp. This optical fiber can be inserted into the biopsy channel of a conventional endoscope. Hence, the app. can be used to perform in situ tissue characterization during endoscopy. The instrument enables the measurement of the decays of entire fluorescence spectra within 15 s with a dynamic range of the spectro-temporal images of up to three orders of magnitude. Luminescence lifetimes from the sub ns up to the ms range can be measured. Spectral and temporal resoln., sensitivity, and dynamic range of the instrumentation were detd. The accuracy of the app. was checked by the measurement of the fluorescence lifetimes of various fluorophores with known lifetimes. For the first time, two-dimensional time-resolved spectra with sub-ns temporal resoln. of tissue fluorescence of the human bladder, the bronchi, and the esophagus taken during endoscopy are presented as a demonstration of performance of the instrumentation. The excitation wavelengths were 337 nm in the case of the bladder and the esophagus and 480 nm in the case of the bronchi. Lifetime contrasts between normal and neoplastic tissue were found in all three organs. The spectral anal. of the fluorescence decays showed that the fluorescence between 370 and 490 nm, excited at 337 nm, consisted in several overlapping spectra. In the case of the esophagus, the contrast between normal and tumoral tissue was inverse in two different spectral bands proving the importance of the choice of the appropriate spectral range for time-resolved autofluorescence measurements for an optimal contrast. The in vivo fluorescence decay of the photosensitizers 5-aminolevulinic acid hexylester hydrochloride-induced protoporphyrin IX was measured in the human bladder and found to be mono-exponential with a lifetime of 15.9 (+-1.2) ns. An in vivo fluorescence lifetime of 8.5 (+-0.8) ns was found in the case of the photosensitizer 5, 10, 15, 20-tetra(m-hydroxyphenyl)chlorin (mTHPC) in the esophagus.

    Keywords: Photomedicine group

    Note:

    opyright 2003 ACS; CAPLUS; AN 1999:628257; Institute of Environmental Engineering,Swiss Federal Institute of Technology of Lausanne (EPFL),Lausanne,Switz. FIELD URL:; Journal; RSINAK; written in English.; 1) Souhami, R; Cancer and its Management, 2nd ed 1995|2) Monnier, P; Lasers Med Sci 1990, 5, 149|3) Jichlinski, P; Lasers Surg Med 1997, 20, 402|4) Wagnieres, G; Photochem Photobiol 1998, 68, 603|5) Bigio, I; Phys Med Biol 1997, 42, 803|6) Richards-Kortum, R; Annu Rev Phys Chem 1996, 47, 555|7) Wagnieres, G; J Fluoresc 1997, 7, 75|8) Schneckenburger, H; Opt Eng (Bellingham) 1992, 31, 995|9) Tang, G; Lasers Surg Med 1989, 9, 290|10) Kohl, M; Appl Phys B: Photophys Laser Chem 1993, 56, 131|11) Cubeddu, R; Photochem Photobiol 1997, 66, 229|12) Spizzirri, P; Lasers Med Sci 1996, 11, 237|13) Ambroz, M; J Photochem Photobiol 1994, 22, 105|14) Andersson-Engels, S; Pharmacology 1992, 3, 66|15) Koenig, F; J Urol (Baltimore) 1998, 159, 1871|16) Saarnak, A; Lasers Med Sci 1998, 13, 22|17) Mordon, S; J Photochem Photobiol B 1992, 13, 307|18) Vaupel, P; Cancer Res 1989, 49, 6449|19) Griffiths, J; Br J Cancer 1991, 64, 425|20) Rye, H; Nucleic Acids Res 1992, 20, 2803|21) Herlin, P; Endoscopy 1983, 15, 4|22) Kocher, O; J Submicrosc Cytol 1981, 13, 267|23) Massaad, L; Bull Cancer 1993, 80, 397|24) Weber, G; Advances in Enzyme Regulation 1977, 15, 53|25) Gottfried, V; Photochem Photobiol 1988, 48, 157|26) Wagnieres, G; Fluorescence Microscopy and Fluorescent Probes 1996, 203|27) Ricchelli, F; J Photochem Photobiol B 1995, 29, 109|28) Lam, S; Chest 1998, 113, 696|29) Vo-Dinh, T; Appl Spectrosc 1997, 51, 58|30) Dhingra, J; Arch Otolaryngol 1996, 122, 1181|31) Schomacker, K; Lasers Surg Med 1992, 12, 63|32) Stone, H; Radiat Res 1993, 136, 422|33) Zilberstein, J; Photochem Photobiol 1997, 65, 1012|34) Brown, J; Cancer Res 1998, 58, 1408|35) Sitnik, T; Br J Cancer 1998, 77, 1386|36) Bambot, S; Trends Biotechnol 1995, 13, 106|37) Moan, J; Photochem Photobiol 1986, 43, 681|38) Scheckenburger, H; Photochem Photobiol 1987, 46, 765|39) Lakowicz, J; Proc Natl Acad Sci USA 1992, 89, 1271|40) Wakita, M; J Biochem 1995, 118, 1151|41) Visser, A; Photochem Photobiol 1981, 33, 35|42) Konig, K; J Photochem Photobiol B 1997, 37, 91|43) Dowling, K; Opt Lett 1998, 23, 810|44) Konig, K; J Photochem Photobiol B 1993, 18, 287|45) Nokubo, M; Biochim Biophys Acta 1988, 939, 441|46) Marriott, G; Biophys J 1991, 60, 1374|47) Szmacinski, H; Sens Actuators B 1995, 29, 16|48) Mizeret, J; PhD thesis No 1839, Swiss Federal Institute of Technology in Lausanne (EPFL) 1998|49) Fleurot, N; Proc SPIE 1984, 491, 374|50) Schiller, N; Opt Commun 1980, 35, 451|51) Hecht, E; Optics, 2nd ed 1987|52) Watanabe, M; Photochem Photobiol 1994, 80, 429|53) Kinoshita, K; Proc SPIE, High Speed Photography, Videography, and Photonics VI 1988, 981, 62|54) Wiedwald, J; Proc SPIE, High-Speed Photography, Videography, and Photonics III 1985, 569, 201|55) Whiteson, A; Proc SPIE, Ultrahigh- and High-Speed Photography, Videography, and Photonics 1991, 1539, 64|56) Nordlund, T; Proc SPIE, Time-Resolved Laser Spectroscopy in Biochemistry 1988, 909, 35|57) Castellano, F; Photochem Photobiol 1998, 67, 179|58) Lakowicz, J; Principles of Fluorescence Spectroscopy 1986|59) O'Connor, D; J Phys Chem 1979, 83, 1333|60) Mizeret, J; Lasers Med Sci 1997, 12, 209|61) Urakami, T; Proc SPIE, High-Speed Photography, Videography, and Photonics IV 1986, 693, 98|62) O'Connor, D; Time-correlated Single Photon Counting 1984|63) Schuitmaker, J; J Photochem Photobiol, B 1996, 34, 3|64) Glanzmann, T; Photochem Photobiol 1998, 67, 596|65) Mellish, R; Opt Lett 1995, 20, 2312

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    Record created on 2007-07-20, modified on 2016-08-08

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