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research article

Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells

Ehrmann, K.  
•
Saillen, N.
•
Vincent, F.  
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2007
Lab on a chip

NMR-microprobes based on solenoids and Helmholtz coils have been microfabricated and NMR-spectra of mammalian cells have successfully been taken. The microfabrication technology developed for these probes consists of three electroplated copper levels for low resistance coils and three SU-8 layers for the integration of microchannels. This technology allows fabricating solenoids, Helmholtz and planar coils on the same wafer. The coils have inner diameters in the range of 160 to 400 microm and detection volumes of 5 to 22 nL. The solenoid and Helmholtz coils show improved RF-field characteristics compared to a planar coil fabricated with the same process. The fabricated solenoid has a particularly low resistance of only 0.46 Omega at 300 MHz. Moreover, it is very sensitive and has a very uniform RF-field, but shows large line width. The Helmholtz coils are slightly less sensitive, but display a far narrower line width, and are therefore a good compromise. With a Helmholtz coil, a SNR of 620 has been measured after one scan on 9 nL pure water. An NMR-microprobe based on a Helmholtz coil has also been used to take spectra of CHO cells that have been concentrated in the sensitive region of the coil with a mechanical filter integrated into the channel.

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Type
research article
DOI
10.1039/b614044k
Web of Science ID

WOS:000244616900011

PubMed ID

17330169

Author(s)
Ehrmann, K.  
Saillen, N.
Vincent, F.  
Stettler, M.  
Jordan, M.  
Wurm, F. M.  
Besse, P. A.  
Popovic, R.
Date Issued

2007

Published in
Lab on a chip
Volume

7

Issue

3

Start page

373

End page

80

Subjects

Animals

•

*Cells

•

Electricity

•

Magnetic Resonance Spectroscopy/*instrumentation

•

Mammals

Note

Microsystems Laboratory, EPFL-Ecole Polytechnique Federale de Lausanne, CH-1015, Lausanne, Switzerland.

Editorial or Peer reviewed

REVIEWED

Written at

EPFL

EPFL units
LBTC  
Available on Infoscience
June 5, 2007
Use this identifier to reference this record
https://infoscience.epfl.ch/handle/20.500.14299/7690
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