Large-scale transient expression in mammalian cells for recombinant protein production

Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been achieved for non-viral delivery methods: transfections can now be done at the 1-10 L scale with mammalian cells grown in suspension. Production of 1-20 mg/L of recombinant protein have been obtained in stirred bioreactors. Modified alphaviruses have provided a fast and efficient expression technology based on viral vectors.


Published in:
Current opinion in biotechnology, 10, 2, 156-9
Year:
1999
ISSN:
0958-1669
Keywords:
Note:
Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland.
Laboratories:




 Record created 2007-06-05, last modified 2018-03-17

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