Large-scale transient expression in mammalian cells for recombinant protein production
Large-scale transient expression from mammalian cells is a new technology. Breakthroughs have been achieved for non-viral delivery methods: transfections can now be done at the 1-10 L scale with mammalian cells grown in suspension. Production of 1-20 mg/L of recombinant protein have been obtained in stirred bioreactors. Modified alphaviruses have provided a fast and efficient expression technology based on viral vectors.
- URL: http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=10209142
Keywords: Adenoviridae/genetics ; Alphavirus/genetics ; Animals ; Cell Culture Techniques ; Cell Line ; Cercopithecus aethiops ; Cloning ; Molecular ; Cricetinae ; Genetic Vectors ; Humans ; Recombinant Proteins/*biosynthesis/genetics ; Vaccinia virus/genetics
Ecole Polytechnique Federale de Lausanne, 1015 Lausanne, Switzerland.
Record created on 2007-06-05, modified on 2016-08-08