000104578 001__ 104578
000104578 005__ 20181203020835.0
000104578 0247_ $$2doi$$a10.1002/gene.20177
000104578 02470 $$2DAR$$a8042
000104578 02470 $$2ISI$$a000235267300001
000104578 037__ $$aARTICLE
000104578 245__ $$aIdentification of evolutionarily conserved regulatory elements in the mouse Fgf8 locus
000104578 269__ $$a2006
000104578 260__ $$c2006
000104578 336__ $$aJournal Articles
000104578 520__ $$aThe secreted signaling molecule fibroblast growth factor 8 (Fgf8) is an essential component of certain embryonic signaling centers including the mid-hindbrain (isthmic) organizer, the first branchial arch (BA1), and the apical ectodermal ridge (AER). In these signaling centers Fgf8 transcripts are expressed in a dynamic and transient fashion, but the mechanism by which this highly specific expression pattern is established remains largely unknown. We used DNA sequence comparisons coupled to transgenic approaches to obtain insight into the structure and function of regulatory elements in the Fgf8 locus. First, a bacterial artificial chromosome (BAC) containing the mouse Fgf8 gene partially rescues the embryonic lethality of Fgf8-deficient mice and controls Fgf8-specific gene expression of a coinjected lacZ reporter transgene. Second, sequence comparison of vertebrate Fgf8 loci revealed evolutionarily highly conserved noncoding sequences that were unexpectedly located mainly 3' of the Fgf8 coding region. Third, in transgenic mice some of these elements were sufficient to target expression to the AER, tail bud, and brain, including the isthmic organizer, indicating that they may represent Fgf8 cis-acting elements. Collectively, these data identify novel regulatory elements of the Fgf8 gene sufficient to drive expression to regions of known Fgf8 activity. genesis 44:1-6, 2006. (c) 2006 Wiley-Liss, Inc.
000104578 700__ $$0240256$$aBeermann, F.$$g169239
000104578 700__ $$aKaloulis, K.
000104578 700__ $$aHofmann, D.
000104578 700__ $$aMurisier, F.
000104578 700__ $$0244404$$aBucher, P.$$g113607
000104578 700__ $$0240661$$aTrumpp, A.$$g168677
000104578 773__ $$j44$$k1$$q1-6$$tGenesis
000104578 909C0 $$0252092$$pGR-BEERMANN$$xU11779
000104578 909C0 $$0252368$$pUPTRU$$xU11160
000104578 909C0 $$0252244$$pGR-BUCHER$$xU11780
000104578 909CO $$ooai:infoscience.epfl.ch:104578$$pSV$$particle
000104578 937__ $$aUPTRU-ARTICLE-2006-007
000104578 970__ $$a13/UPTRU
000104578 973__ $$aOTHER$$rREVIEWED$$sPUBLISHED
000104578 980__ $$aARTICLE