000101531 001__ 101531
000101531 005__ 20181203020752.0
000101531 0247_ $$2doi$$a10.1016/j.cub.2006.07.059
000101531 02470 $$2ISI$$a000240719800030
000101531 037__ $$aARTICLE
000101531 245__ $$aSequential Protein Recruitment in C. elegans Centriole Formation
000101531 269__ $$a2006
000101531 260__ $$c2006
000101531 336__ $$aJournal Articles
000101531 520__ $$aFormation of the microtubule-based centriole is a poorly understood process that is crucial for duplication of the centrosome, the principal microtubule-organizing center of animal cells . Five proteins have been identified as being essential for centriole formation in Caenorhabditis elegans: the kinase ZYG-1, as well as the coiled-coil proteins SAS-4, SAS-5, SAS-6, and SPD-2 . The relationship between these proteins is incompletely understood, limiting understanding of how they contribute to centriole formation. In this study, we established the order in which these five proteins are recruited to centrioles, and we conducted molecular epistasis experiments expanding on earlier work. We find that SPD-2 is loaded first and is needed for the centriolar localization of the four other proteins. ZYG-1 recruitment is required thereafter for the remaining three proteins to localize to centrioles. SAS-5 and SAS-6 are recruited next and are needed for the presence of SAS-4, which is incorporated last. Our results indicate in addition that the presence of SAS-5 and SAS-6 allows diminution of centriolar ZYG-1. Moreover, astral microtubules appear dispensable for the centriolar recruitment of all five proteins. Several of these proteins have homologs in other metazoans, and we expect the assembly pathway that stems from our work to be conserved.
000101531 700__ $$aDelattre, M.
000101531 700__ $$aCanard, C.
000101531 700__ $$g168676$$aGönczy, P.$$0243625
000101531 773__ $$j16$$tCurrent Biology$$k18$$q1844-1849
000101531 909C0 $$xU11155$$0252248$$pUPGON
000101531 909CO $$pSV$$particle$$ooai:infoscience.tind.io:101531
000101531 937__ $$aUPGON-ARTICLE-2007-001
000101531 973__ $$rREVIEWED$$sPUBLISHED$$aEPFL
000101531 980__ $$aARTICLE