Lentiviral-mediated gene transfer to model triplet repeat disorders
This chapter describes the potential use of viral-mediated gene transfer in the central nervous system as a new strategy in developing animal models of neurodegenerative diseases. To illustrate the approach, procedures for the production of lentiviral vectors encoding polyQ proteins are provided, as well as methods for the determination of viral titers, in vitro infection, and basic protocols for in vivo studies in rodents.
Keywords: Animals ; Cell Line ; Female ; Gene Transfer Techniques ; Genetic Diseases ; Inborn/ genetics ; Humans ; Immunohistochemistry ; Lentivirus/ genetics ; Pregnancy ; Rats ; Rats ; Sprague-Dawley ; Trinucleotide Repeats/ genetics
Institute of Neuroscience, School of Life Sciences, Swiss Federal Institute of Technology Lausanne, Switzerland.
Record created on 2007-03-09, modified on 2016-08-08