000101281 001__ 101281
000101281 005__ 20181203020749.0
000101281 0247_ $$2doi$$a10.1016/j.biomaterials.2003.10.030
000101281 022__ $$a0142-9612
000101281 02470 $$2ISI$$a000220558200056
000101281 037__ $$aARTICLE
000101281 245__ $$aIn vivo calcium deposition on polyvinyl alcohol matrix used in hollow fiber cell macroencapsulation devices
000101281 260__ $$c2004
000101281 269__ $$a2004
000101281 336__ $$aJournal Articles
000101281 500__ $$aDivision of Surgical Research and Gene Therapy Center, CHUV, Lausanne University Medical School, Lausanne, Switzerland.
000101281 520__ $$aThe encapsulation of genetically modified cells represents a promising approach for the delivery of therapeutic proteins. The functionality of the device is dependent on the characteristics of the biomaterials, the procedures used in its confection and the adaptability of the encapsulated cells in the host. We report conditions leading to the development of calcifications on the polyvinyl alcohol (PVA) matrix introduced in hollow fiber devices for the encapsulation of primary human fibroblasts implanted in mice. The manufacturing procedures, batches of PVA matrix and cell lineages were assessed for their respective role in the development of the phenomenon. The results showed that the calcification is totally prevented by substituting phosphate-buffer saline with ultra-pure sterile water in the rinsing procedure of the matrix. Moreover, a positive correlation was found, when comparing two fibroblast cell lineages, between the level of lactate dehydrogenase (LDH) activity measured in the cells and the degree of calcium deposition. Higher LDH activity may decrease calcium depositions because it generates in the device a more acidic microenvironment inhibiting calcium precipitation. The present study defines optimized conditions for the encapsulation of primary human fibroblasts in order to avoid potentially detrimental calcifications and to allow long-term survival of encapsulated cells.
000101281 6531_ $$aAnimals
000101281 6531_ $$aBiocompatible Materials/ chemistry
000101281 6531_ $$aCalcinosis/etiology/ pathology
000101281 6531_ $$aCalcium/ chemistry
000101281 6531_ $$aCell Culture Techniques/ methods
000101281 6531_ $$aCell Line
000101281 6531_ $$aCell Transplantation/ methods
000101281 6531_ $$aForeign-Body Reaction/etiology/ pathology
000101281 6531_ $$aHumans
000101281 6531_ $$aMale
000101281 6531_ $$aMaterials Testing
000101281 6531_ $$aMice
000101281 6531_ $$aMice
000101281 6531_ $$aInbred BALB C
000101281 6531_ $$aMice
000101281 6531_ $$aNude
000101281 6531_ $$aPolyvinyl Alcohol/adverse effects/ chemistry
000101281 6531_ $$aMice
000101281 6531_ $$aMice
000101281 700__ $$aSchwenter, F.
000101281 700__ $$0241464$$aBouche, N.$$g150219
000101281 700__ $$aPralong, W. F.
000101281 700__ $$0240206$$aAebischer, P.$$g104359
000101281 773__ $$j25$$k17$$q3861-8$$tBiomaterials
000101281 909C0 $$0252067$$pLEN$$xU10457
000101281 909CO $$ooai:infoscience.tind.io:101281$$pSV$$particle
000101281 937__ $$aLEN-ARTICLE-2004-004
000101281 970__ $$a17/LEN
000101281 973__ $$aEPFL$$rREVIEWED$$sPUBLISHED
000101281 980__ $$aARTICLE